Summary
Studies with insulin delivery devices have shown that denaturation of dissolved proteins at hydrophobic interfaces is a basic obstacle to long-term insulin stability in pumps. This study shows that polypropylene glycol/polyethylene glycol block polymers prevent both the adsorption of dissolved proteins to hydrophobic interfaces and the resultant aggregation. At a concentration of 0.001% (w/v), the block polymer Genapol PF-10 stabilises insulin solutions over a wide range of concentrations. Insulin solutions thus stabilised are at present being clinically tested. The effectiveness of molecular variants of Genapol PF-10 to stabilise other proteins (human γ-globulin, myoglobin and serum albumin) is presented also.
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Thurow, H., Geisen, K. Stabilisation of dissolved proteins against denaturation at hydrophobie interfaces. Diabetologia 27, 212–218 (1984). https://doi.org/10.1007/BF00273809
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DOI: https://doi.org/10.1007/BF00273809