Summary
The non-obese diabetic mouse is thought to be one of the best available animal models for human Type 1 (insulin-dependent) diabetes. By 51Cr release assay we investigated cell-mediated cytotoxicity to the islet cells of Balb/C mice, natural killer activity, and antibody-dependent cell-mediated cytotoxicity activity of spleen lymphocytes from pre-diabetic non-obese diabetic mice. The cell-mediated cytotoxicity to islet cells of non-obese diabetic mice was significantly higher than that of control ICR mice. In contrast, natural killer and antibody-dependent cell-mediated cytotoxicity activities of the spleen cells from the non-obese diabetic mice were significantly lower than those of ICR mice spleen cells. These results suggest that lymphocytes from non-obese diabetic mice were sensitized to the antigen of islet cells and that the non-specific cellular immunity of non-obese mice was reduced. They suggest also that this immune islet cell-killing mechanism may play an important role in the pathogenesis of diabetes in non-obese diabetic mice.
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Makino S, Kunimoto K, Muraoka Y, Mizushima Y, Katagiri K, Tochino Y (1980) Breeding of a non-obese diabetic strain of mice. Exp Anim 29: 1–13
Tochino Y, Kanaya T, Makino S (1982) Genetics of NOD mice. In: Mimura G, Baba S, Goto Y, Köbberling J (eds) Clinico-genetic genesis of diabetes mellitus. Excerpta Medica, Amsterdam, pp 285–291
Toyota T, Kataoka S, Sato J, Fujita H, Hayashida Y, Goto Y, Kumagai K (1982) Islet-cell antibody and immunologic aspect of NOD mice. In: Mimura G, Baba S, Goto Y, Köbberling J (eds) Clinico-genetic genesis of diabetes mellitus. Excerpta Medica, Amsterdam, pp 185–192
Takei I, Maruyama T, Taniyama M, Maruyama H, Kataoka K, Ishii T (1982) Islet-cell surface antibodies and lymphocyte antibodies in the spontaneously diabetic NOD mouse. In: Alberti KGMM, Ogada T, Aluoch JA, Mngola EN (eds) 11th Congress of the International Diabetes Federation, Nairobi, p 204 (Abstract)
Kataoka S, Satoh J, Fujita H, Toyota T, Suzuki R, Itoh K, Kumagai K (1983) Immunologic aspect of nonobese diabetic (NOD) mouse. Abnormalities of cellular immunity. Diabetes 32: 247–253
Lernmark Å, Kanatsuna T, Potzelt C, Diakoumis K, Carroll R, Rubenstein AH, Steiner DF (1980) Antibodies directed against the pancreatic islet cell membrane. Diabetologia 19: 445–451
Huang SW, Maclaren NK (1976) Insulin-dependent diabetes: a disease of autoaggression. Science 192: 64–66
Charles MA, Suzuki M, Waldeck N, Dodson LE, Slater L, Ong K, Kershner A, Buckingham B, Golden M (1983) Immune islet killing mechanisms associated with insulin-dependent diabetes: in vitro expression of cellular and antibody-dependent islet cell cytotoxicity in humans. J Immunol 130: 1189–1194
Dobersen MN, Scharf JE, Ginsberg-Fellner F, Notkins AL (1980) Cytotoxic autoantibodies to beta cells in the serum of patients with insulin-dependent diabetes mellitus. N Engl J Med 303: 1493–1498
Kanatsuna T, Lernmark Å, Rubenstein AH, Steiner DF (1981) Block in insulin release from column-perifused pancreatic β-cell induced by cell surface antibodies and complement. Diabetes 30: 231–234
Maruyama T, Takei I, Matsuba I, Tsuruoka A, Taniyama M, Ikeda Y, Kataoka K, Abe M, Matsuki S (1984) Cell-mediated cytotoxic islet cell surface antibodies to human pancreatic beta cells. Diabetologia 26: 30–33
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Maruyama, T., Takei, I., Taniyama, M. et al. Immunological aspect of non-obese diabetic mice: immune islet cell-killing mechanism and cell-mediated immunity. Diabetologia 27 (Suppl 1), 121–123 (1984). https://doi.org/10.1007/BF00275666
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DOI: https://doi.org/10.1007/BF00275666