Summary
This review demonstrates the following points:1. By glucose tolerance test and the determination of non-fasting blood sugar values, mice of KK strain (a Japanese strain) have been shown to be in a diabetic state. 2. KK mice have greater than normal body weights, and moderate obesity. 3. Pancreatic insulin activity and adenohypophyseal growth hormone content of KK mice are greater than those of C75BL mice. 4. The pancreas of KK mice presents many striking changes, such as the hypertrophy and hyperplasia of the islets, the hypertrophy and degranulation of B cells, the abundance of B cell ribosomes and endoplasmic reticulum, the enlargement of the Golgi areas of B cells, and the diminution of the zinc content of insular cells. 5. In other organs (eg. adenohypophysis, liver, adrenal and parathyroid) of KK mice, many changes are also observed. The above features of KK mice are discussed in relation to the presumed metabolic disorder in the mice.
Résumé
1. Un état diabétique est démontré chez des souris d'une souche japonaise, la souche KK, mis en évidence par une élévation du sucre sanguin et par une diminution de la tolérance au glucose. 2. Le poids corporel des souris KK dépasse la normale; il s'agit d'une obésité modérée. 3. L'activité insulinique du pancréas et le contenu en hormone de croissance de l'hypophyse de souris KK dépassent ceux observés chez des souris C57BL. 4. Histologiquement, on trouve chez les souris KK une hypertrophie et une hyperplasie des îlots de Langerhans, une hypertrophie et une dégranulation des cellules B, une abondance de ribosomes et de réticulum endoplasmique des cellules B, un développement marqué de la région de Golgi des mêmes cellules, et une diminution du contenu en zinc des cellules insulaires. D'autres anomalies ont également été observées dans d'autres organes des souris KK, plus particulièrement pour l'hypophyse, le foie, les surrénales et la parathyroïde.
Zusammenfassung
1. Bei Mäusen des KK-Stammes (japanische Zucht) ist mit Bestimmung von Glucosetoleranz und Blutzuckerwerten ein diabetischer Zustand nachweisbar. 2. Das Körpergewicht von KK-Mäusen liegt über der Norm, und es besteht eine leichte Fettsucht. 3. Die Insulinaktivität des Pankreas und der Gehalt der Hypophyse an Wachstumshormon sind bei KK-Mäusen höher als bei C57BL-Mäusen. 4. Das Pankreas der KK-Mäuse zeigt auffallende Veränderungen, wie Hypertrophie und Degranulierung derβ-Zellen, reichlich Ribosomen und endoplasmatisches Reticulum in denβ-Zellen, sowie starke Ausbildung des Golgiapparates und Verminderung des Zinkgehaltes der Inselzellen. 5. Auch andere Organe (z.B. Adenohypophyse, Leber, Nebenniere und Nebenschilddrüse) der KK-Mäuse weisen Veränderungen auf.
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References
Barrnett, R., R. Marshall andA. Seligman: Histochemical demonstration of insulin in the islet of Langerhans. Endocrinology57, 419–438 (1955).
Best, C., C. Jephcott andD. Scott: Insulin in tissue other than the pancreas. Amer. J. Physiol.100, 285–295 (1932).
Bielschowsky, M., andF. Bielschowsky: The New Zealand strain of obese mice. Their response to stilboestrol and to insulin. Aust. J. exp. Biol. med. Sci.34, 181–198 (1956).
Bjorkman, N., C. Hellerström andB. Hellman: The ultrastructure of the islets of Langerhans in normal and obese-hyperglycemic mice. Z. Zellforsch.,58, 803–819 (1963).
Elftman, H.: A chrom-alum fixative for the pituitary. Stain Technol.32, 25–28 (1957).
Gomori, G.: The distribution of phosphatase in normal organs and tissues. J. cell. comp. Physiol.17, 71–83 (1941).
— Observations with differential stains on human islet of Langerhans. Amer. J. Path.17, 395–406 (1941).
— An improved histochemical technic for acid phosphatase. Stain Technol.25, 81, (1950).
Hellerström, C., andB. Hellman: The islets of Langerhans in yellow obese mice. Metabolism12, 527–536 (1963).
Jones, E.: Spontaneous hyperplasia of the pancreatic islets associated with glucosuria in hybrid mice. The Structure and Metabolism of the Pancreatic Islets, edited byBrolin S.E., B. Hellman andH. Knutson, Oxford, Pergamon Press, pp. 189–193, 1964.
Kondo, K., K. Nozawa, T. Tomida andK. Ezaki: Inbred strains resulting from Japanese mice. Bull. exp. Animals6, 107–112 (1957).
Lazarow, A., C. Bauer andA. Lindall: Protein synthesis in islet tissue. The Structure and Metabolism of the Pancreatic Islets, edited byBrolin, S.E., B. Hellman andH. Knutson. Oxford, Pergamon Press, pp. 203–221, 1964.
Lazarus, S.: Demonstration of glucose-6-phosphatase in mammalian pancreas. Proc. Soc. exp. Biol.101, 819–822 (1959).
Lewis, U.J., E.V. Cheever andW.P. Vanderlaan:Studies on the growth hormone of normal and dwarf mice. Endocrinology76, 210–215 (1965).
Like, A., J. Steinke E. Jones andG. Cahiil: Pancreatic studies in mice with spontaneous diabetes. Amer. J. Path.46, 621–644 (1965).
Maeda, T., T. Fujiwara andK. Hironari: Histochemical demonstration of zinc. Bull. Kobe Med. College5, 97–99 (1954).
Nakamura, M.: A diabetic strain of the mouse. Proc. Japan. Acad.38, 348–352 (1962).
— Cytological and histological studies on the pancreatic islets of a diabetic strain of the mouse. Z. Zellforsch.65, 340–349 (1965).
— Estimation of adenohypophyseal growth hormone content in the diabetic “KK” mouse strain by acrylamide gel electrophoresis. Proc. Japan. Acad.42, 512–516 (1966).
—, andK. Yamada, Jr.: A further study of the diabetic (KK) strain of the mouse. F1 and F2 offsprings of the cross between KK and C57BL mice. Proc. Japan. Acad.39, 489–493 (1963).
— — Enzymorphological studies on the pancreatic islets of a diabetic (KK) strain of the mouse. Z. Zellforsch.66, 396–404 (1965).
Ogita, Z.: Disc electrophoresis. Metabolism and Disease2, 419–435 (1965).
Padykula, H., andE. Herman: Factors affecting activity of adenosinetriphosphatase and other phosphatases as measured by histochemical techniques. J. Histochem. Cytochem.3, 161–169 (1955a).
— — Specificity of histochemical method for adenosinetriphosphatase. J. Histochem. Cytochem.3, 170–195 (1955b).
Palade, G.: A study of fixation for electron microscopy. J. exp. Med.95, 285–298 (1952).
Tsuchida, I.: Studies on the mechanism of hyperglycemia and obesity in KK strain of the mice. Japan Diabetic Soc.9, 67–84 (1966).
Wachstein, M.: Histochemical staining reactions of the normally functioning and abnormal kidney. J. Histochem. Cytochem.3, 246–270 (1956).
Wrenshall, G., S. Andrus andJ. Mayer: High levels of pancreatic insulin coexistent with hyperglycemia and degranulation of beta cells in mice with the hereditary obese-hyperglycemic syndrome. Endocrinology56, 335–340 (1954).
Yamada, K., Jr.: The chemical cytology of the mouse parathyroid gland. Z. Zellforsch.65, 805–813 (1965).
- M.Nakamura and K.Yamashita: Light and electron microscopic studies on the adenohypophysis of a Diabetic (KK) strain of the mouse. In press (1966).
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Nakamura, M., Yamada, K. Studies on a diabetic (KK) strain of the mouse. Diabetologia 3, 212–221 (1967). https://doi.org/10.1007/BF01222198
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DOI: https://doi.org/10.1007/BF01222198