Summary
The intestine is a major site of cholesterol synthesis and produces apolipoprotein B-48, which is critical for intestinal cholesterol absorption and secretion. The purpose of this study was to examine postprandial changes in apolipoprotein B-48 in diabetes. Six non-insulin-dependent diabetic patients and six non-diabetic control subjects were given a high-fat meal (1300 kcal) and blood samples were taken pre- and postprandially, from which the triglyceride-rich lipoprotein fraction was isolated by ultracentrifugation (density<1.006 g/ml). Apolipoprotein B-48 was separated on 4–15% gradient gels and quantified as a percentage of the fasting concentration by densitometric scanning. Total protein, triglyceride and cholesterol in the triglyceride-rich lipoprotein fraction, blood glucose, and serum insulin were also measured. Diabetic patients exhibited a postprandial triglyceride-rich apolipoprotein B-48 profile significantly different from that of control subjects (p<0.05). The triglyceride and total protein concentration in the triglyceride-rich lipoprotein fraction mirrored the post-prandial profile and apolipoprotein B-48 in both groups. Significantly different patterns for triglyceride (p<0.02) and total protein (p<0.05) following the fat-rich meal were observed in the two groups. Fasting and postprandial triglyceride-rich lipoprotein cholesterol and total apolipoprotein B were significantly higher in diabetic patients than in control subjects (p<0.05). Since apolipoprotein B-48 is the structural protein of intestinally-derived lipoprotein particles, these studies suggest an abnormality in intestinal lipoprotein metabolism in diabetes.
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Abbreviations
- TRL:
-
Triglyceride-rich lipoprotein
- Apo-B48:
-
apolipoprotein B-48
- apo-B100:
-
apolipoprotein B-100
- apo E:
-
apolipoprotein E
- VLDL:
-
very low density lipoprotein
- NIDDM:
-
non-insulin-dependent diabetes
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Curtin, A., Deegan, P., Owens, D. et al. Alterations in apolipoprotein B-48 in the postprandial state in NIDDM. Diabetologia 37, 1259–1264 (1994). https://doi.org/10.1007/BF00399800
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DOI: https://doi.org/10.1007/BF00399800