Summary
To study the effects of chronic osmotic diuresis which were not associated with hyperglycaemia on the rat kidney, osmotic diuresis was induced by i. v. infusion of urea. A 5 mol/l urea solution was continuously infused at a rate of 100 ml · kg−1 · day−1 on the basis of body weight on day 0. Duration of infusion was 2, 6, 10 or 14 days. Control rats received continuously infused Ringer's solution. Urea-treated groups developed osmotic diuresis (urine flow = about 0.04 ml · min −1 · 100 g body weight−1) comparable to that in rats with experimental diabetes mellitus induced by i. v. streptozotocin (55 mg/kg), however urea-induced osmotic diuresis was not associated with blood glucose level increases. Compared with their controls, rats receiving urea for 2–14 days had markedly increased kidney weight. Rats receiving urea for 10 days showed greatest kidney weight increase, 0.565 ± 0.044 g/100 g body weight (mean ± SD), representing a 53% increase compared with the control (0.369 ± 0.034 g/100 g body weight). Kidney weight was associated with increases in kidney protein content. In contrast, none of control kidney weight values differed significantly from day 0 values (=normal rats; 0.387 ± 0.028 g/100 g body weight). Creatinine clearance values in urea-treated groups were also higher than those in controls. The maximum value, 0.65 ± 0.17.ml · min−1 · 100 g body weight−1, was recorded in the 14-day group and was significantly higher than the corresponding control value (0.34 ± 0.07 ml · min−1 · 100 g body weight−1) (p <0.001). Urea clearance values were also significantly higher in urea-treated groups than in respective controls. This study suggests that osmotic diuresis may induce renal hypertrophy/hyperplasia and glomerular hyperfiltration immediately after development of diabetes.
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Abbreviations
- DM:
-
Diabetes mellitus
- ID:
-
inner diameter
- OD:
-
outer diameter
References
Stalder G, Schmid R (1959) Severe functional disorders of glomerular capillaries and renal hemodynamics in treated diabetes mellitus during childhood. Ann Pediatr 193: 129–138
Mogensen CE, Andersen MJF (1973) Increased kidney size and glomerular filtration rate in early juvenile diabetes. Diabetes 22: 706–712
Christiansen JS, Gammelgaard J, Frandsen M, Parving HH (1981) Increased kidney size, glomerular filtration rate and renal plasma flow in short-term insulin-dependent diabetics. Diabetologia 20: 451–456
Mogensen CE, Christensen CK, Vittinghus E (1983) The stages in diabetic renal disease. With emphasis on the stage of incipient diabetic nephropathy. Diabetes 32 [Suppl 2]: 64–78
Seyer-Hansen K (1976) Renal hypertrophy in streptozotocin-diabetic rats. Clin Sci Mol Med 51: 551–555
Brenner BM, Hostetter TH, Olson JL, Rennke HG, Venkatachalam MA (1981) The role of glomerular hyperfiltration in the initiation and progression of diabetic nephropathy. Acta Endocrinol 97 [Suppl 242]: 7–10
Østerby R, Gundersen HJG (1981) Fast accumulation of basement membrane material and the rate of morphological changes in acute experimental diabetic glomerular hypertrophy. Diabetologia 18: 493–500
Seyer-Hansen K, Hansen J, Gundersen HJG (1980) Renal hypertrophy in experimental diabetes. A morphometric study. Diabetologia 18: 501–505
Wiseman M, Viberti GC (1983) Kidney size and glomerular filtration rate in type 1 (insulin-dependent) diabetes mellitus revisited. Diabetologia 25: 530
Hostetter TH, Brenner BM (1982) The case for intrarenal hypertension in the initiation and progression of diabetic and other glomerulopathies. Am J Med 72: 375–380
Steiger E, Vars HM, Dudrick SJ (1972) A technique for longterm intravenous feeding in unrestrained rats. Arch Surg 104: 330–332
Lowry OH, Rosebrough NJ, Farr AL, Randell RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–271
Brown DM, Andres GA, Hostetter TH, Mauer SM, Price R, Venkatachalam MA (1982) Kidney complications. Diabetes 31 [Suppl 1]: 71–81
Seely JF, Dirks JH (1969) Micropuncture study of hypertonic mannitol diuresis in the proximal and distal tubule of the dog kidney. J Clin Invest 48: 2330–2340
Sladek CD, Knigge KM (1977) Osmotic control of vasopressin release by rat hypothalamo-neurohypophyseal explants in organ culture. Endocrinology 101: 1834–1838
Ishikawa S, Saito T, Yoshida S (1980) The effect of osmotic pressure and angiotensin II on arginine vasopressin release from guinea pig hypothalamo-neurohypophyseal complex in organ culture. Endocrinology 106: 1571–1578
Zerbe RL, Robertson GL (1983) Osmoregulation of thirst and vasopressin secretion in human subjects: effect of various solutes. Am J Physiol 244: E607-E614
Kauker ML, Lassiter WE, Gottschalk CW (1970) Micropuncture study of effects of urea infusion on tubular reabsorption in the rat. Am J Physiol 219: 45–50
Edwards BR, Novakova A, Sutton RAL, Dirks JH (1973) Effect of acute urea infusion on proximal tubular reabsorption in the dog kidney. Am J Physiol 224: 73–79
Halliburton IW (1968) The effect of unilateral nephrectomy and of diet on the composition of the kidney. In: Nowinski WW, Goss RJ (eds) Compensatory renal hypertrophy. Academic Press, New York, pp 101–128
Jones G, Lee K, Swaminathan R (1985) Glomerular filtration response to acute protein load. Lancet 12: 838
Dhaene M, Sabot JP, Philippart Y, Doutrelepont JM, Vanherweghem JL (1987) Effect of acute protein loads of different sources on glomerular filtration rate. Kidney Int 32 [Suppl 22]: S-25–S-28
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Ogino, Y., Okada, S. & Ota, Z. Effects of chronic, urea-induced osmotic diuresis on kidney weight and function in rats. Diabetologia 37, 225–231 (1994). https://doi.org/10.1007/BF00398047
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DOI: https://doi.org/10.1007/BF00398047