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Effects of early insulin treatment on ultrastructural changes of glomeruli in diabetic rats revealed by the quick-freezing and deep-etching method

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Summary

The three-dimensional ultrastructure of glomerular basement membrane (GBM) and mesangial matrix (MM) at an early stage of streptozotocin (STZ)-induced diabetes mellitus in rats was examined by the quick-freezing and deep-etching method. In diabetic rats, the GBM inner layer was diffusely enlarged and the meshwork structure not only in the GBM middle layer but also in the MM became markedly irregular due to the rupture of fine fibrils. This irregularity and enlargement of the mesh pores in diabetic rats developed during the experimental period and was significantly different from results in control rats. Insulin treatment from 1 week after STZ injection had significant effects in preventing the ultrastructural changes in the GBM and MM. It is suggested that early insulin treatment has significant effects in preventing size barrier disturbance of GBM and MM in STZ-induced diabetes.

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Abbreviations

GBM:

Glomerular basement membrane

MM:

mesangial matrix

STZ:

streptozotocin

QF-DE:

quick-freezing and deep-etching

LDM:

longest dimension of meshpores

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Authors and Affiliations

  1. Department of Internal Medicine, Kitasato University School of Medicine, Sagamihara, Japan

    T. Moriya, K. Tanaka & Y. Yajima

  2. Department of Anatomy, Yamanashi Medical University, Yamanashi, Japan

    S. Ohno & Y. Fujii

Authors
  1. T. Moriya
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  2. S. Ohno
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  3. K. Tanaka
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  4. Y. Fujii
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  5. Y. Yajima
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Moriya, T., Ohno, S., Tanaka, K. et al. Effects of early insulin treatment on ultrastructural changes of glomeruli in diabetic rats revealed by the quick-freezing and deep-etching method. Diabetologia 39, 632–640 (1996). https://doi.org/10.1007/BF00418534

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  • DOI: https://doi.org/10.1007/BF00418534

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