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Lipoprotein composition in NIDDM: effects of dietary oleic acid on the composition, oxidisability and function of low and high density lipoproteins

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Summary

Oxidation of low density lipoprotein (LDL) plays an important role in the pathogenesis of atherosclerosis and is related to the fatty acid composition which is altered in diabetes mellitus. This study examines the relationship between the fatty acid composition of LDL and high density lipoprotein (HDL) and lipoprotein oxidation. A group of nine non-insulin-dependent diabetic (NIDDM) patients were compared to seven healthy control subjects before and after a high monounsaturated diet. Lipoproteins were isolated and oxidisability was measured by conjugated diene formation and lipid peroxide analysis. Serum HDL cholesterol was significantly lower in the diabetic patients. LDL cholesteryl ester linoleic acid in the diabetic patients was significantly higher at baseline and decreased after diet (p<0.05) while oleic acid increased in both diabetic and non-diabetic subjects (p<0.05). HDL cholesteryl ester oleic acid was lower in the diabetic patients compared with control subjects (p<0.05) before diet and it increased significantly after diet (p<0.05). LDL lipid peroxides and conjugated diene formation were related to LDL glycation (r=0.46, p<0.05 and r=0.49, p<0.05, respectively). Both decreased following diet (lipid peroxides for diabetic patients from 476±30 to 390±20 nmol/mg protein p<0.05 and for control subjects from 350±36 to 198±30 nmol/mg protein p<0.05). HDL conjugated diene formation decreased in both groups after diet but only significantly in the control group (55.4±7.5 to 53.2±6.7 nmol/mg protein for diabetic patients and 45.8±6.4 to 31.6±4.8 nmol/mg protein p<0.05 for control subjects). There was a positive correlation between LDL lipid peroxide formation and percentage of cholesteryl ester linoleic acid in LDL from diabetic patients (r=0.61, p<0.05) and control subjects (r=0.91, p<0.01). Fatty acid composition of LDL was reflected in the composition of HDL. In the presence of HDL lipoprotein peroxidation decreased. This decrease in lipoprotein peroxidation was positively related to the percentage of linoleic acid in LDL (r=0.71, p<0.05). This study confirms the close relationship between the fatty acid composition of LDL and HDL and demonstrates the importance of the fatty acid composition of the cholesteryl ester fraction in relation to LDL oxidation in diabetes. Linoleic acid in HDL appears to be a protecting factor against oxidation.

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Abbreviations

BHT:

Butylated hydroxytoluene

EDTA:

ethyl-enediaminetetraacetic acid

TBARS:

thiobarbituric reacting substances

HPLC:

high performance liquid chromatography

MDA:

malondialdehyde

HbA1c :

glycated haemoglobin

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Authors and Affiliations

  1. Department of Clinical Medicine, Trinity College Dublin, Dublin, Ireland

    E. Dimitriadis, D. Owens & G. R. Tomkin

  2. The Adelaide Hospital, Dublin, Ireland

    M. Griffin

  3. Department of Biochemistry, The Royal College of Surgeons in Ireland, Dublin, Ireland

    P. Collins, A. Johnson & G. R. Tomkin

Authors
  1. E. Dimitriadis
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  2. M. Griffin
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  3. P. Collins
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  4. A. Johnson
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  5. D. Owens
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  6. G. R. Tomkin
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Dimitriadis, E., Griffin, M., Collins, P. et al. Lipoprotein composition in NIDDM: effects of dietary oleic acid on the composition, oxidisability and function of low and high density lipoproteins. Diabetologia 39, 667–676 (1996). https://doi.org/10.1007/BF00418538

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