Summary
Pancreatic glands of 22-day-old rat foetuses or 0–12 day old rats were incubated in a Krebs-Ringer phosphate-buffered salt solution, and the insulin secretion measured at a lower (0.6 mg/ml) or a higher (3 mg/ml) glucose concentration in the medium. Stimulation of the insulin release by glucose could not be detected until the second postnatal day, when there was a marked increase in the amount of hormone secreted into the medium. Since the presence of insulin has been demonstrated in the B-cells of rat foetuses, the data suggest that in this species the cellular mechanism for insulin synthesis becomes manifest considerably earlier than that for glucose-regulated insulin release.
Résumé
Les glandes pancréatiques de foetus de rats âgés de 22 jours ou de rats âgés de 0–12 jours ont été incubées dans une solution tampon au phosphate de Krebs Ringer, et la sécrétion d'insuline a été mesurée pour une faible concentration de glucose (0.6 mg/ml) ou pour une concentration de glucose plus élevée (3 mg/ml) dans le milieu. La stimulation de la libération d'insuline par le glucose ne pouvait pas être décelée jusqu'au deuxième jour postnatal, où il y avait une nette augmentation de la quantité d'hormone sécrétée dans le milieu. Puisque la présence d'insuline a été démontrée dans les cellules B de foetus de rats, les données suggèrent que dans cette espèce le mécanisme cellulaire de la synthèse de l'insuline devient manifeste beaucoup plus tôt que celui de la libération d'insuline régulée par le glucose.
Zusammenfassung
Das Pankreas von 22 Tage alten Rattenfoeten oder 0–12 Tage alten Ratten wurde in einer Krebs-Ringer-Phosphat-Puffer-Lösung inkubiert, und die Insulin-Sekretion bei einer niedrigeren (0.6 mg/ml) oder höheren (3 mg/ml) Glucosekonzentration der Lösung gemessen. Stimulation der Insulinfreisetzung durch Glucose konnte bis zum 2. postnatalen Tag nicht festgestellt werden, an dem ein bemerkenswerter Anstieg des in die Lösung sezernierten Hormons eintrat. Da die Gegenwart von Insulin in den B-Zellen von Rattenfoeten gezeigt wurde, lassen die Werte vermuten, daß sich bei dieser Spezies der Zellmechanismus für Insulin-Synthese beträchtlich früher manifestiert als die glucoseregulierte Insulinfreisetzung.
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This investigation was supported by grants from the Medical Faculty of Uppsala, the Swedish Medical Research Council (B69-12X-109-05A) and the U.S. Public Health Service (AM-12535).
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Asplound, K., Westman, S. & Hellersteöm, C. Glucose stimulation of insulin secretion from the isolated pancreas of foetal and newborn rats. Diabetologia 5, 260–262 (1969). https://doi.org/10.1007/BF01212095
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DOI: https://doi.org/10.1007/BF01212095