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The utilisation of d-galactonate and d-2-oxo-3-deoxygalactonate by Escherichia coli K-12

Biochemical and genetical studies

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Abstract

  1. 1.

    Escherichia coli K-12 mutants unable to grow on d-galactonate have been isolated and found to be defective in either galactonate dehydratase, 2-oxo-3-deoxygalactonate 6-phosphate aldolase or devoid of both of these enzymes and of 2-oxo-3-deoxygalactonate kinase.

  2. 2.

    2-Oxo-3-deoxygalactonate kinase and 2-oxo-3-deoxygalactonate 6-phosphate aldolase are still induced by galactonate in mutants lacking galactonate dehydratase, suggesting that galactonate rather than a catabolic product of galactonate is the inducer of the galactonate catabolic enzymes. Synthesis of the enzymes is subject to glucose catabolite repression.

  3. 3.

    Mutants defective in 2-oxo-3-deoxygalactonate 6-phosphate aldolase accumulate 2-oxo-3-deoxygalactonate 6-phosphate when exposed to galactonate and this compound causes general growth inhibition.

  4. 4.

    Secondary mutants that no longer show this inhibition fail to make 2-oxo-3-deoxygalactonate 6-phosphate due to additional defects in galactonate transport, galactonate dehydratase, 2-oxo-3-deoxygalactonate kinase or a putative promoter mutation that prevents formation of these enzymes.

  5. 5.

    A spontaneous mutant capable of growth on 2-oxo-3-deoxygalactonate has been isolated. It has two genetically distinct mutations. One permits constitutive formation of the galactonate catabolic enzymes and the other allows the uptake of 2-oxo-3-deoxygalactonate. Neither mutation on its own permitted growth on 2-oxo-3-deoxygalactonate.

  6. 6.

    Genes specifying the various galactonate catabolic enzymes have been located at min 81.7 on the E. coli K-12 linkage map and probably constitute an operon. The gene sequence in this region was shown to by: pyrE uhp dgo dnaA.

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Abbreviations

ODGalO kinase:

2-oxo-3-deoxygalactonate kinase

ODGalOP aldolase:

2-oxo-3-deoxygalactonate 6-phosphate aldolase

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Authors and Affiliations

  1. Department of Biochemistry, University of Leicester, University Road, LE1 7RH, Leicester, England

    Ronald A. Cooper

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  1. Ronald A. Cooper
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Where no steroisomeric form is indicated, the sugars are of the d series

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Cooper, R.A. The utilisation of d-galactonate and d-2-oxo-3-deoxygalactonate by Escherichia coli K-12. Arch. Microbiol. 118, 199–206 (1978). https://doi.org/10.1007/BF00415730

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  • DOI: https://doi.org/10.1007/BF00415730

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