Abstract
A nystatin-resistant mutant of Rhodotorula gracilis was obtained by treatment of the wild strain cells with N-methyl-N-nitro-N-nitrosoguanidine and selected on agar plates containing 150 μg nystatin/ml. Three important transport functions of the plasma membrane of mutant cells: the accumulation of monosaccharides, the generation and maintenance of the pH-gradient and of the membrane potential, as well as the cell respiration were insensitive to at least 10-5 M nystatin. This concentration of nystatin inhibited completely all these processes in wild strain cells. Analysis of cellular sterols revealed a defect of ergosterol biosynthesis in the mutant, which was localized at the last oxidative step between 5,6-dihydroergosterol and ergosterol.
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Finkelstein A, Holz R (1973) Aqueous pores created in thin lipid membranes by polyene antibiotics nystatin and amphotericin B. In: Eisenman G (ed) Membranes, vol 2. Dekker, New York, pp 377–408
Fryberg M, Oehlschläger AC, Unrau AM (1974) Sterol biosynthesis in antibiotic-resistant yeast: Nystatin. Arch Biochem Biophys 160:83–89
Hauer R, Höfer M (1978) Evidence for interactions between the energydependent transport of sugars and the membrane potential in the yeast Rhodotorula gracilis (Rhodosporidium toruloides). J Membrane Biol 43: 335–349
Hedenström M von, Höfer M (1979) The effect of nystatin on active transport in Rhodotorula glutinis (gracilis) is restricted to the plasma membrane. Biochim Biophys Acta 555:169–174
Heller KB, Höfer M (1975) Temperature dependence of the energylinked monosaccharide transport across the cell membrane of Rhodotorula gracilis. J Membrane Biol 21:261–271
Höfer M, Misra PC (1978) Evidence for the proton/sugar symport in the yeast Rhodotorula glutinis (gracilis). Biochem J 172:15–22
Hoogevest P van, De Kruijff B (1978) Effect of amphotericin B on cholesterol containing liposomes of egg phosphatidylcholine and didocosenoyl phosphatidylcholine. A refinement of the model for the formation of pores by amphotericin B in membranes. Biochim Biophys Acta 511:397–407
Kaneko H, Hosohara M, Tanaka M, Itoh T (1976) Lipid composition of 30 species of yeast. Lipids 11:837–840
Kinsky SC, Luse SA, van Dennen LLM (1966) Interaction of polyene antibiotics with natural and artificial membrane systems. Fed Proc 25:1503–1510
Kotyk A, Höfer M (1965) Uphill transport of sugars in the yeast Rhodotorula gracilis. Biochim Biophys Acta 102:410–422
Kruijff B De, Demel RA (1974) Polyene antibiotic-sterol interactions in membranes of Archeloplasma laidlawii cells and lecithin liposomes. III. Molecular structure of the polyene antibiotic-cholesterol complexes. Biochim Biophys Acta 339:57–70
Lampen JO (1966) Interference by polyenic antifungal antibiotics (especially nystatin and filipin) with specific membrane functions. Symp Soc Gen Microbiol 16:111–130
Longley RP, Rose AH, Knights BA (1968) Composition of the protoplast membrane from Saccharomyces cerevisiae. Biochem J 108:401–412
Misra PC, Höfer M (1975) An energy-linked proton extrusion across the cell membrane of Rhodotorula gracilis. FEBS Lett 52:95–99
Shaw WHC, Jefferies JP (1953) The determination of ergosterol in yeast. III. Corrections for irrelevant absorption in solutions of ergosterol. Analyst 78:519–523
Stadtman TC (1957) Preparation and assay of cholesterol and ergosterol. In: Colowick SP, Kaplan NO (eds) Methods of enzymology, vol III. Academic Press Inc, New York, pp 392–394
Thiele OW, Dreysel J, Hermann D (1972) The “free” lipids of two different strains of hydrogen-oxidizing bacteria in relation to their growth phases. Eur J Biochem 29:224–235
Wells MA, Dittmer JC (1963) The use of Sephadex for the removal of nonlipid contaminants from lipid extracts. Biochem 2:1259–1263
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Höfer, M., Thiele, O.W., Huh, H. et al. A nystatin-resistant mutant of Rhodotorula gracilis. Transport properties and sterol content. Arch. Microbiol. 132, 313–316 (1982). https://doi.org/10.1007/BF00413381
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DOI: https://doi.org/10.1007/BF00413381