Abstract
The broadspectrum herbicide glyphosate (N-[phosphonomethyl]glycine), an inhibitor of the shikimate pathway enzyme 5-enolpyruvyl-shikimic acid-3-phosphate (EPSP)-synthase, inhibits the growth of Aerobacter aerogenes and causes the excretion of shikimic acid-3-phosphate. A strain of A. aerogenes, resistant to inhibition of growth by glyphosate, was isolated and found to have a glyphosate-insensitive EPSP-synthase and to no longer excrete shikimic acid-3-phosphate in the presence of glyphosate. Partial identity of EPSP-synthases from the glyphosate-sensitive and-resistant A. aerogenes strains was demonstrated by immunological procedures.
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Abbreviations
- EPSP-synthase:
-
5-enolpyruvylshikimic acid-3-phosphate synthase (EC 2.5.1.19; 3-phosphoshikimate 1-carboxyvinyltransferase)
References
Amrhein N, Deus B, Gehrke P, Steinrücken HC (1980) The site of the inhibition of the shikimate pathway by glyphosate. II. Interference of glyphosate with chorismate formation in vivo and in vitro. Plant Physiol 66:830–834
Amrhein N, Deus B, Gehrke P, Holländer H, Schab J, Schulz A, Steinrücken HC (1981) Interference of glyphosate with the shikimate pathway. Proc Plant Growth Regul Soc America 8:99–106
Amrhein N, Holländer-Czytko H, Leifeld J, Schulz A, Steinrücken HC, Topp H (1982) Inhibition of the shikimate pathway by glyphosate. In: Boudet AM, Ranjeva R (eds) Journées internationales d'ètudes et assemblées gènèrales. Bulletin de Liaison, vol 11. Groupe Polyphenols, Toulouse, pp 21–30
Amrhein N, Johänning D, Schab J, Schulz A (1983) Biochemical basis for glyphosate-tolerance in a bacterium and a plant tissue culture. FEBS-Letters 157:191–196
Boocock MR, Coggins JR (1983) Kinetics of 5-enolpyruvylshikimate-3-phosphate synthase inhibition by glyphosate. FEBS-Letters 154:127–133
Comai L, Sen LC, Stalker DM (1983) An altered aroA gene product confers resistance to the herbicide glyphosate. Science 221:370–371
Gibson MI, Gibson F (1964) Preliminary studies on the isolation and metabolism of an intermediate in aromatic biosynthesis: chorismic acid. Biochem J 90:248–261
Holländer-Czytko H, Amrhein N (1983) subcellular compartmentation of shikimic acid and phenylalanine in buckwheat cell suspension cultures grown in the presence of shikimate pathway inhibitors. Plant Sci Lett 29:89–96
Ouchterlony Ö (1949) Antigen-antibody reactions in gels. Acta Path Microbiol Scand 26:507–515
Rogers SG, Brand LA, Holder SB, Sharps ES, Brackin MJ (1983) Amplification of the aroA gene from Escherichia coli results in tolerance to the herbicide glyphosate. Appl Environmental Microbiol 46:37–43
Steinrücken HC (1982) Zur Wirkungsweise des Herbizides Glyphosat: einfluß auf die 5-Enolpyruvylshikimisäure-3-phosphat-Synthase aus Aerobacter aerogenes 62-1. Doctoral Dissertation, Ruhr-Universität, Bochum
Steinrücken HC, Amrhein N (1980) The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthase. Biochem Biophys Res Commun 94:1207–1212
Weiler EW, Zenk MH (1976) Radioimmunoassay for the determination of digoxin and related compounds in Digitalis lanata. Phytochemistry 15:1537–1545
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Schulz, A., Sost, D. & Amrhein, N. Insensitivity of 5-enolpyruvylshikimic acid-3-phosphate synthase to glyphosate confers resistance to this herbicide in a strain of Aerobacter aerogenes . Arch Microbiol 137, 121–123 (1984). https://doi.org/10.1007/BF00414451
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DOI: https://doi.org/10.1007/BF00414451