Skip to main content
Log in

Subcellular distribution of yeast invertase isoenzymes

  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Homogenates from yeast cells contain 1% or less of sedimentable invertase activity. Sedimentability is equally low in homogenates from cells repressed or derepressed with regard to invertase secretion. Intracellularly, the mannanprotein form of invertase is largely localized in vacuoles whereas the small isoenzyme is largely present in the soluble cell fraction. These findings indicate that vesicles are not involved in the secretion of invertase. A soluble mode of invertase secretion is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Beteta, P., Gascòn, S.: Localization of invertase in yeast vacuoles. FEBS Letters 13, 297–300 (1971)

    Google Scholar 

  • Cortat, M., Matile, Ph., Kopp, F.: Intracellular localization of mannan synthetase activity in budding baker's yeast. Biochem. biophys. Res. Commun. 53, 482–489 (1973)

    Google Scholar 

  • Cortat, M., Matile, Ph., Wiemken, A.: Isolation of glucanasecontaining vesicles from budding yeast. Arch. Mikrobiol. 82, 189–205 (1972)

    Google Scholar 

  • Gascòn, S., Neumann, P., Lampen, J.O.: Comparative study of the purified internal and external invertases from yeast. J. biol. Chem. 243, 1573–1577 (1968)

    Google Scholar 

  • Gascòn, S., Ottolenghi, P.: Invertase isoenzymes and their localization in yeast. C. R. Lab. Carlsberg 36, 85–93 (1967)

    Google Scholar 

  • Holley, R.A., Kidby, D.K.: Role of vacuoles and vesicles in extra-cellular enzyme secretion from yeast. Canad. J. Microbiol. 19, 113–117 (1973)

    Google Scholar 

  • Lampen, J.O.: Yeast invertase. In: The enzymes, P. D. Boyer, ed., vol. V, pp. 291–316. New York: Academic Press 1971

    Google Scholar 

  • Lampen, J.O., Kuo, S.-C., Cano, F.R., Tkacz, J.S.: Structural features in synthesis of external enzymes by yeast. Proc. 4th Int. Fermentation Symposium, Kyoto, pp. 122–128 (1972)

  • Matile, Ph., Wiemken, A.: The vacuole as the lysosome of the yeast cell. Arch. Mikrobiol. 56, 148–155 (1967)

    Google Scholar 

  • Maurer, H.R.: Disk-Elektrophorese. Berlin: de Gruyter 1968

    Google Scholar 

  • Meyer, J., Matile, Ph.: Regulation of isoenzymes and secretion of invertase in baker's yeast. Biochem. Physiol. Pflanzen (in press)

  • Tkacz, J.S., Lampen, J.O.: Surface distribution of invertase on growing Saccharomyces cells. J. Bact. 113, 1073–1075 (1973)

    Google Scholar 

  • Wiemken, A.: Eigenschaften der Hefevakuole. Thesis Nr. 4340, ETH Zürich (1969)

  • Wiemken, A., Nurse, P.: The vacuole as a compartment of amino acid pools in yeast. Proc. Third Int. Specialized Symp. on Yeasts, part II, pp. 331–347 (1973)

  • van der Wilden, W., Matile, Ph., Schellenberg, M., Meyer, J., Wiemken, A.: Vacuolar membranes: Isolation from yeast cells. Z. Naturforsch. 28c, 416–421 (1973)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Meyer, J., Matile, P. Subcellular distribution of yeast invertase isoenzymes. Arch. Microbiol. 103, 51–55 (1975). https://doi.org/10.1007/BF00436329

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00436329

Key words

Navigation