Abstract
Ultraviolet (UV) sensitivity and photoreactivation of blue-green algae Cylindrospermum sp., Plectonema boryanum, spores of Fischerella muscicola and algal virus (cyanophage) LPP-1 were studied. The survival value after UV irradiation of filaments of Cylindrospermum sp. and Virus LPP-1 showed exponential trend and these were comparatively sensitive towards UV than F. muscicola and P. boryanum. Photoreactivation of UV-induced damage occurred in black, blue, green, yellow, red and white light in Cylindrospermum sp., however only black, blue and white light were capable of photorepair of UV-induced damage in P. boryanum, spores of F. muscicola and virus LPP-1 in infected host alga. Pre-exposure to yellow and black light did not show photoprotection. The non-heterocystous and nitrogen fixation-less mutants of Cylindrospermum sp. were not induced by UV and their spontaneous mutation frequency was not affected after photoreactivation. The short trichome mutants of P.boryanum were more resistant towards UV.
The occurrence of photoreactivation of UV-induced killing in wide range of light in Cylindrospermum sp. is the first report in organisms.
References
Adams, M. H.: Bacteriophages. New York: Interscience 1959
Allen, M. B., Arnon, D. I.: Studies on nitrogen fixing blue-green algae. I. Growth and nitrogen fixation by Anabaena cylindrica Lemm. Plant Physiol. 30, 366–372 (1955)
Harm, W.: Dark repair of photorepairable UV lesions in Escherichia coli. Mutation Res. 6, 25–35 (1968)
Jagger, J., Stafford, R. S.: Evidence for two mechanism of photoreactivation in Escherichia coli B. Biophys. J. 5, 75–88 (1965)
Rupert, C. S., Harm, W.: Reactivation after photobiological damage. In: Advances in radiation biology, Vol. 2. L. G. Augenstein, R. Mason, M. Zelle, eds., pp. 1–81. New York: Academic Press 1966
Safferman, R. S., Morris, M. E.: Algal virus: isolation. Science 140, 679–680 (1963)
Safferman, R. S., Morris, M. E.: Growth characteristics of the blue-green algal virus LPP-1. J. Bact. 88, 771–775 (1964)
Saito, N., Werbin, H.: Purification of a blue-green algal DNA-photoreactivating enzyme. An enzyme requiring light as a physical cofactor to perform its catalytic function. Biochemistry 9, 2610–2619 (1970)
Singh, P. K.: Ultraviolet sensitivity and genetics of blue-green algae and their viruses. Ph. D. Thesis, Banaras Hindu University, India (1967)
Singh, P. K.: Algicidal effect of 2,4-dichlorophenoxy acetic acid on blue-green alga Cylindrospermum sp. Arch. Microbiol. 97, 69–72 (1974)
Singh, H. N.: Effect of acriflavine on ultraviolet sensitivity of normal, ultraviolet sensitive and ultraviolet resistant strains of a blue-green alga Anacystis nidulans. Rad. Bot. 8, 355–361 (1968)
Singh, R. N., Singh, P. K.: Mutation with loss of nitrogen fixation, heterocysts and spore production in the blue-green alga Cylindrospermum. Abst. All India Conference of Microbiologists, Baroda (1968)
Singh, R. N., Singh, P. K.: Ultraviolet damage, modifications and repair of blue-green algae and their viruses. In: Taxonomy and biology of blue-green algae, T. V. Desikachary, ed., pp. 246–257. Madras, India: University of Madras 1972
Singh, R. N., Singh, S. P., Singh, P.K.: Genetic regulation of nitrogen fixation in blue-green algae. In: Taxonomy and biology of blue-green algae, T. V. Desikachary, ed., pp. 264–268. Madras, India: University of Madras 1972
Van Baalen, C.: The effect of ultraviolet irradiation on a coccoid blue-green alga: survival, photosynthesis, and photoreactivation. Plant Physiol. 43, 1689–1695 (1968)
Werbin, H., Rupert, C. S.: Presence of photoreactivating enzyme in blue-green algal cells. Photochem. Photobiol. 7, 225–230 (1968)
Witkin, E. M.: Ultraviolet-induced mutation and DNA repair. Ann. Rev. Microbiol. 23, 487–514 (1969a)
Witkin, E. M.: The role of DNA repair and recombination in mutagenesis. Proc. XII Intern. Congr. Genetics, Vol. 3, pp. 225–245 (1969b)
Wu, J. H., Lewin, R. A., Werbin, H.: Photoreactivation of UV-irradiated blue-green algal virus LPP-1. Virology 31, 657–664 (1967)
Wulff, D. L., Rupert, C. S.: Disappearance of thymine photodimer in ultraviolet irradiated DNA upon treatment with a photoreactiving enzyme from baker's yeast. Biochem. biophys. Res. Commun. 7, 237–240 (1962)
Zhevner, V. D., Shestakov, S. V.: Studies on the ultraviolet sensitive mutants of blue-green alga Synechocystis aquatilis Sanv. Arch. Mikrobiol. 86, 349–360 (1972)
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Singh, P.K. Photoreactivation of UV-irradiated blue-green algae and algal virus LPP-1 . Arch. Microbiol. 103, 297–302 (1975). https://doi.org/10.1007/BF00436364
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DOI: https://doi.org/10.1007/BF00436364