Abstract
The raw extracts of a series of microorganisms were screened for the presence of acetyl-coenzyme A: arylamine N-acetyltransferase (AAAT) using a radioactive assay with 3H-acetyl-coenzyme A and aniline as substrates. Enzyme activities were primarily detected in the soluble fractions of Bacillus and Nocardia species, and in some further soil organisms. Only strains of Bacillus cereus were able to acetylate 4-nitroaniline and 3,5-dimethyl-4-nitroaniline. The fermentation conditions for the production of the enzyme were optimized. The AAAT from one strain of Bacillus cereus was purified 24-fold and characterized.
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Abbreviations
- AAAT:
-
acetyl-coenzyme A: arylamine N-acetyltransferase
- AcP:
-
acetylphosphate
- CoA:
-
coenzyme A
- EDTA:
-
ethylenediaminetetra-acetic acid
- PTA:
-
phosphotransacetylase
References
Andres HH (1982) Reinigung und Charakterisierung der Acetyl-Coenzym A: Arylamin N-Acetyltransferase aus Taubenleber und deren Verwendung zur Bestimmung von Enzymen, Substraten und Inhibitoren. Thesis, Technical University of Munich
Andres HH, Kolb HJ, Weiss L (1983a) Purification and physicalchemical properties of acetyl-CoA: arylamine N-acetyltransferase from pigeon liver. Biochim Biophys Acta 746:182–192
Andres HH, Kolb HJ, Schreiber RJ, Weiss L (1983b) Characterization of the active site, substrate specificity and kinetic properties of acetyl-CoA: arylamine N-acetyltransferase from pigeon liver. Biochim Biophys Acta 746:193–201
Bachofer R, Lingens F, Schäfer W (1975) Conversion of aniline into pyrocatechol by a Nocardia sp.; incorporation of oxygen-18. FEBS Lett 50:288–290
Bamberger E (1925) Studien über Arylazide. 5. Mitteilung. Liebig's Ann Chem 443:207
Barenholz Y, Gatt S (1972) Long chain base-acetyl coenzyme A acetyltransferase from the microsomes of Hansenula ciferri. I. Isolation and properties. J Biol Chem 247:6827–6833
Barenholz Y, Edelman I, Gatt S (1974) Amine: acetyl coenzyme A acetyltransferase from the soluble fraction of Hansenula ciferri: Isolation and properties. Biochim Biophys Acta 358:262–274
Cartwright NJ, Cain RB (1959) Bacterial degradation of the nitrobenzoic acids. 2. Reduction of the nitro group. Biochem J 73:305–314
Davies J, Smith DI (1978) Plasmid determined resistance to antimicrobial agents. Annu Rev Microbiol 32:469–518
Engelhardt G, Wallnöfer P, Fuchsbichler G, Baumeister W (1977) Bacterial transformations of 4-chloroaniline. Chemosphere 2/3: 85–92
Glowinski IB, Weber WW (1982) Genetic regulation of aromatic amine N-acetylation in inbred mice. J Biol Chem 257:1424–1430
Gollamudi R, Rackley RJ, Autian J (1983) A new substrate for the measurement of N-acetyltransferase activity. Enzyme 30:155–161
Katz J, Liebermann I, Barker HA (1953) Acetylation of amino acids by enzymes of Clostridium kluyveri. J Biol Chem 200:417–429
Kaufman DD, Plimmer JR, Klingebiel UI (1973) Microbial oxidation of 4-chloroaniline. J Agric Food Chem 21:127–132
Münzner R, Mutschler E, Rummel M (1967) Über die mikrobiologische Umwandlung N-haltiger Substrate. 1. Mitteilung: Über die mikrobiologische Acetylierung von Aminen durch Cordyceps militaris. Planta Med 15:97–103
Paul RC, Ratledge C (1971) N-acetylanthranilic acid biosynthesis in Aerobacter aerogenes and Escherichia coli. Biochim Biophys Acta 230:451–461
Rummel M (1967) Über mikrobiologische Umwandlung stick-stoffhaltiger organischer Verbindungen. Thesis, Johannes Gutenberg University, Mainz
Shaw WV (1975) Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria. In: Hash JH (ed). Methods Enzymol 43:737–755
Tabor H, Mehler AH, Stadtman ER (1953) The enzymatic acetylation of amines. J Biol Chem 204:127–138
Tacker M, McIsaac WM, Creaven PJ (1970) Metabolism of tyramine-1-14C by the rat. Biochem Pharmacol 19:2763–2773
Wallnöfer P, Engelhardt G, Fuchsbichler G (1976) Das Verhalten von Anilinderivaten als Abbauprodukte chemischer Pflanzenschutzmittel im Boden. Bayer Landwirtsch Jahrb 53:309–317
Wallnöfer PR, Ziegler W, Engelhardt G, Rothmeier H (1978) Tranformation of dinitrophenol-herbicides by Azotobacter sp. Chemosphere 12:967–972
Weber WW, Cohen SN, Steinberg MS (1968) Purification and properties of N-acetyltransferase from mammalian liver. Ann NY Acad Sci 157:734–741
You IS, Bartha R (1982) Metabolism of 3,4-dichloroaniline by Pseudomonas putida. J Agric Food Chem 30:274–277
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Hasmann, M.J., Seidl, P.H., Engelhardt, G. et al. Acetyl-coenzyme A: arylamine N-acetyltransferases in microorganisms: screening and isolation of an enzyme from Bacillus cereus . Arch. Microbiol. 146, 275–279 (1986). https://doi.org/10.1007/BF00403229
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DOI: https://doi.org/10.1007/BF00403229