Abstract
Uptake of tungstate by growing cells was unaffected by the presence of molybdate in Clostridium cylindrosporum, whereas in C. acidiurici the accumulation was decreased by molybdate at 10-6 mol/l tungstate and higher concentrations. The labelling pattern of soluble proteins by 185W-tungsten indicated after gel chromatography the presence of three different tungstoproteins in both bacteria. Formate dehydrogenase activity always eluted at a maximum of tungsten labelling. The incorporation of tungsten into formate dehydrogenase containing fractions and a possible tungsten-binding-storage protein was independent of the presence of excess molydate pointing to a genuine role for tungstate in these bacteria.
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Wagner, R., Andreesen, J.R. Accumulation and incorporation of 185W-tungsten into proteins of Clostridium acidiurici and Clostridium cylindrosporum . Arch. Microbiol. 147, 295–299 (1987). https://doi.org/10.1007/BF00463491
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DOI: https://doi.org/10.1007/BF00463491