Abstract
In Methanobacterium thermoautotrophicum a corrinoid-carrying membrane protein complex has been found, to which a tentative role in methane formation has been ascribed. To test this hypothesis representatives from different orders of methanogenic bacteria were examined for membrane-bound cobamides. These species differed in cell carbon precursor, in methane precursor, in occurrence of cytochromes and of the enzyme CO dehydrogenase, and in the systematic position (Methanobacteriales, Methanomicrobiales). All methanogenic bacteria contained cobamides in the membranes in amounts of about 60 nmol/g cell dry weight, in addition to different amounts of cobamides in the soluble cell fraction. The only central metabolic reaction obviously common to all of these methanogens was methyl coenzyme M reduction to CH4. It is concluded that the membrane corrinoid participates in this energy-conserving reaction.
Sulfate-reducing and acetogenic bacteria were included in this survey. They contained different amounts of cobamides in the soluble cell fraction but not in the membrane, a possible exception being Acetobacterium woodii.
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References
Ankel-Fuchs D, Thauer RK (1986) Methane formation from methyl coenzyme M in a system containing methyl-CoM reductase, component B, and reduced cobalamin. Eur J Biochem 156:171–177
Ankel-Fuchs D, Hüster R, Mörschel E, Albracht SPJ, Thauer RK (1986) Structure and function of methylcoenzyme M reductase and of factor F 430 in methanogenic bacteria. System Appl Microbiol 7:383–387
Bak F, Widdel F (1986) Anaerobic degradation of indolic compounds by sulfate-reducing enrichment cultures, and description of Desulfobacterium indolicum gen. nov., sp. nov. Arch Microbiol 146:170–176
Balch WE, Magrum LJ, Fox GE, Wolfe RS, Woese CR (1979) Methanogens: A reevaluation of a unique bacterial group. Microbiol Rev 43:260–296
Bergmeyer HU, Gawehn K, Grassl M (1974) Glycerinaldehyd-3-phosphat-dehydrogenase. In: Bergmeyer HU (ed) Methoden der enzymatischen Analyse, vol 1. Verlag Chemie, Weinheim, pp 495–496
Blaut M, Gottschalk G (1985) Evidence for a chemiosmotic mechanism of ATP synthesis in methanogenic bacteria. Trends Biochem Sci 10:486–489
Bott MH, Eikmanns B, Thauer RK (1985) Defective formation and/or utilization of carbon monoxide in H2/CO2 fermenting methanogens dependent on acetate as carbon source. Arch Microbiol 143:266–269
Brandis-Heep A, Gebhardt NA, Thauer RK, Widdel F, Pfennig N (1983) Anaerobic acetate oxidation to CO2 by Desulfobacter postgatei. I. Demonstration of all enzymes required for the operation of the citric acid cycle. Arch Microbiol 136:222–229
Daniels L, Luchs G, Thauer RK, Zeikus JG (1977) Carbon monoxide oxidation by methanogenic bacteria. J Bacteriol 132:118–126
Diekert G, Weber B, Thauer RK (1980) Nickel dependence of factor F430 content in Methanobacterium thermoautotrophicum. Arch Microbiol 127:273–278
Diekert G, Fuchs G, Thauer RK (1985) Properties and function of carbon monoxide dehydrogenase from anaerobic bacteria. In: Poole RK, Dow CS (eds) Microbial gas metabolism. Mechanistic, metabolic and biotechnical aspects. Academic Press, London, pp 115–130
Dolphin D (1982) B12, vol 1+2. Wiley, New York
Eden G, Fuchs G (1983) Autotrophic CO2 fixation in Acetobacterium woodii. II. Demonstration of enzymes involved. Arch Microbiol 135:68–73
Ellefson WE, Wolfe RS (1981) Component C of the methylreductase system of Methanobacterium. J Biol Chem 256:4259–4262
Ellefson WL, Whitman WB, Wolfe RS (1982) Nickel-containing factor F 430: chromophore of the methylreductase of Methanobacterium. Proc Natl Acad Sci USA 79:3707–3710
Eikmanns B, Thauer RK (1985) Evidence for the involvement and role of corrinoid enzyme in methane formation from acetate in Methanosarcina barkeri. Arch Microbiol 142:175–179
Ferry JG, Wolfe RS (1977) Nutritional and biochemical characterization of Methanospirillum hungatii. Appl Environ Microbiol 34:371–376
Friedrich W (1975) Vitamin B12 und verwandte Corrinoide. In: Ammon R, Dirscherl W (eds) Fermente, Hormone, Vitamine, vol 3/2. Thieme, Stuttgart
Fuchs G (1986) CO2 fixation in acetogenic bacteria: variations on a theme. FEMS Microbiol Rev 39:181–213
Fuchs G, Stupperich E (1986) Carbon assimilation pathways in Archaebacteria. System Appl Microbiol 7:364–369
Goa J (1953) A microbiuret method for protein determination of total protein in cerebrospinal fluid. Scand J Clin Invest 5:218–222
Gottschalk G (1985) Bacterial metabolism, 2nd edn. Springer, Berlin Heidelberg New York, pp 250–253
Holder U, Schmidt DE, Stupperich E, Fuchs G (1985) Autotrophic synthesis of activated acetic acid from two CO2 in Methanobacterium thermoautotrophicum. III. Evidence for common one-carbon precursor pool and the role of corrinoid. Arch Microbiol 141:229–238
Jussofie A, Gottschalk G (1986) Further studies on the distribution of cytochromes in methanogenic bacteria. FEMS Microbiol Lett 37:15–18
Kengen SWM, Poirot CM, Keltjens JT, Vogels GD (1986) Involvement of a corrinoid enzyme in methyl group transfer from methyl-THMP to coenzyme M. In: Duine JA, Verseveld HW van (eds) Abstracts of the 5th International Symposium on Microbial Growth on C1 Compeounds. Free University Press, Amsterdam, p 25
Krzycki J, Zeikus JG (1980) Quantification of corrinoids in methanogenic bacteria. Curr Microbiol 3:243–245
Kühn W, Fiebig K, Hippe H, Mah RA, Huser BA, Gottschalk G (1983) Distribution of cytochromes in methanogenic bacteria. FEMS Microbiol Lett 20:407–410
Länge S, Fuchs G (1987) Autotrophic synthesis of activated acetic acid from CO2 in Methanobacterium thermoautotrophicum. Synthesis from tetrahydromethanopterin-bound C1 units and carbon monoxide. Eur J Biochem 163:147–154
Lezius AG, Barker HA (1965) Corrinoid compounds of Methanobacillus omelianski. I. Fractionation of the corrinoid compounds and identification of factor III and factor III coenzyme. Biochem 4:510–518
Ljungdahl L, Irion E, Wood HG (1966) Role of corrinoids in the total synthesis of acetate from CO2 by Clostridium thermoaceticum. Fed Proc Am Sec Exp Biol 25:1642–1648
Lorowitz WH, Bryant MP (1984) Petostreptococcus productus strain that grows rapidly with CO as the energy source. Appl Environ Microbiol 47:961–964
Miller TL, Wolin MJ (1985) Methanosphaera stadtmaniae gen. nov., sp. nov.: a species that forms methane by reducing methanol with hydrogen. Arch Microbiol 141:116–122
Ossmer R, Mund T, Hartzell PL, Konheiser U, Kohring GW, Klein A, Wolfe RS, Gottschalk G, Mayer F (1986) Immunocytochemical localization of component C of the methylreductase system in Methanococcus voltae and Methanobacterium thermoautotrophicum. Proc Natl Acad Sci USA 83:5789–5792
Pfaltz A, Jaun B, Fässler A, Eschenmoser A, Jaenchen R, Gilles HH, Diekert G, Thauer RK (1982) Zur Kenntnis des Faktors F430 aus methanogenen Bakterien: Struktur des porphinoiden Ligandsystems. Helvetica Chimica Acta 65:828–865
Savéant JM, DeTacconi N, Lexa D, Zickler J (1979) Electrochemistry of vitamin B12 equilibria genetics and mechanisms in the B 12a-B 12r-B 12s oxido-reduction. In: Friedrich W (ed) Vitamin B12. De Gruyter, Berlin, pp 203–212
Schauder R, Eikmanns B, Thauer RK, Widdel F, Fuchs G (1986) Acetate oxidation to CO2 in anaerobic bacteria via a novel pathway not involving reactions of the citric acid cycle. Arch Microbiol 145:162–172
Schönheit P, Moll J, Thauer RK (1980) Growth parameters (K s, K s, µmax, Y s) of Methanobacterium thermoautotrophicum. Arch Microbiol 127:59–65
Schulz H, Fuchs G (1986) Cobamide-containing membrane protein complex in Methanobacterium thermoautotrophicum. FEBS Lett 198:279–283
Stupperich E, Kräutler B (1987) Pseudo vitamin B12 and 5-hydroxybenzimidazolyl-cobamide are the predominant corrinoids of methanogenic bacteria. FEBS Lett (in press)
Stupperich E, Steiner I, Rühlemann M (1986) Isolation and analysis of bacterial cobamides by HPLC. Anal Biochem 155:365–370
Stupperich E, Steiner I, Eisinger H-J (1987) Substitution of Co α-(5-hydroxybenzimidazolyl)cobamide (Factor III) by vitamin B12 in Methanobacterium thermoautotrophicum. J Bacteriol (in press)
Van der Meijden P, Tebrömmelstroet BW, Poirot CM, van der Drift C, Vogels GD (1984) Purification and properties of methanol-5-hydroxybenzimidazolylcobamide methyltransferase from Methanosarcina barkeri. J Bacteriol 160:629–635
Van der Meijden P, van der Drift C, Vogels GD (1985) Methanol conversion in methanogenic and acetogenic bacteria. J Microbiol Serol 51:454
Van de Wijngaard WMH, van der Drift C, Vogels GD (1986) Methane formation from methanol by Methanosphaera stadtmaniae. In: Duine JA, Verseveld HW van (eds) Abstracts of the 5th International Symposium on Microbial Growth on C1 Compounds. Free University Press, Amsterdam, p 24
Widdel F, Pfennig N (1981) Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. I. Isolation of new sulfate-reducing bacteria enriched with acetate from saline environments. Arch Microbiol 129:395–400
Wolfe RS (1985) Unusual coenzymes of methanogenesis. Trends Biochem Sci 10:396–399
Wood HG, Ragsdale SW, Pezacka E (1986) The acetyl-CoA pathway of autotrophic growth. FEMS Microbiol Rev 39:345–362
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Dangel, W., Schulz, H., Diekert, G. et al. Occurrence of corrinoid-containing membrane proteins in anaerobic bacteria. Arch. Microbiol. 148, 52–56 (1987). https://doi.org/10.1007/BF00429647
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DOI: https://doi.org/10.1007/BF00429647