Abstract
Partial DNA sequencing of a genomic clone of the archaebacterium Halobacterium halobium, which hydridized with an avian v-myc probe, showed especially the presence, in the organism of one of the conserved regions through myb, myc and adenovirus E1a oncogenes. The archaebacterial deduced amino acid sequence displayed significant homology with the v-myc gene product. In accordance with the partial DNA sequencing which assured a sufficient homology to have similar epitopes, a protein having a molecular weight of 70,000 and possessing high antigenicity with a polyclonal antiserum against avian v-myc protein was isolated and purified from H. halobium extracts. The purified v-myc like protein stimulated in vitro DNA synthesis carried out by the alpha like DNA polymerase of H. halobium.
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References
Alitalo K, Ramsay G, Bishop JM, Pfeifer SO, Colby WW, Levinson AD (1983a) Identification of nuclear proteins encoded by viral and cellular myc oncogenes. Nature 306:274–277
Alitalo K, Bishop IM, Smith DH, Chen EY Colby WW, Levinson AD (1983b) Nucleotide sequence of the v-myc oncogene of avian retrovirus MC 29. Proc Natl Acad Sci USA 80:100–104
Colby WW, Chen EY, Smith DH, Levinson AD (1983) Identification and nucleotide sequence of a human locus homologous to the v-myc oncogene of avian myelocytomatosis virus MC29. Nature 301:722–725
Daniels JC, Gupta R, Doolittle WF (1985) Transcription and excision of a large intron in the tRNAtrp gene of an archeabacterium, Halobacterium volcanii. J Biol Chem 260:3132–3134
Doolittle RF (1981) Similar amino acid sequences: chance or common ancestry? Science 214:149–159
Ebert K, Goebel W (1985) Conserved and variable regions in the chromosomal and extrachromosomal DNA of halobacteria. Mol Gen Genet 200:96–102
Hamlyn PH, Rabbitts TH (1983) Translocation joins c-myc and immunoglobulin gamma 1 genes in a Burkitt lymphoma revealing a third exon in the c-myc oncogene. Nature 304:135–139
Kjelms J, Garrett A (1985) An intron in the 23S ribosomal RNA gene of the archaebacterium Desulfurococcus mobilis. Nature 318:675–677
Kohiyama M, Nakayama M, Ben-Mahrez K (1986a) DNA polymerase and primase-reverse transcriptase from Halobacterium halobium. Syst Appl Microbiol 7:79–82
Kohiyama M, Ben-Mahrez K, Krycève-Martinerie C, Sougakoff W, Perbal B (1986b) DNA sequences of archaebacteria related to v-myc or v-myb oncogenes. In: Kandler O (ed) Archaebacteria '85. Fischer, Stuttgart New York, p 402
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, New York
Moritz A, Lankat-Buttgereit B, Gross HJ, Goebel W (1985) Common structural features of the genes for two stable RNAs from Halobacterium halobium. Nucl Acid Res 13:31–43
Mount SM (1982) A catalogue of splice junction sequences. Nucl Acid Res 10:459–472
Nakayama M, Kohiyama M (1985) An alpha-like DNA polymerase from Halobacterium halobium. Eur J Biochem 152:293–297
Persson H, Leder P (1984) Nuclear localization and DNA binding properties of a protein expressed by human c-myc oncogene. Science 225:718–721
Ralston R, Bishop JM (1983) The protein products of the myc and myb oncogenes and adenovirus E1a are structurally related. Nature 306:803–806
Rasmussen RD, Staprans SI, Shaw SB, Spector DH (1985) Sequences in human cytomegalovirus which hybridize with the avian retrovirus oncogene v-myc are G+C rich and do not hybridize with the human c-myc gene. Mol Cell Biol 5:1525–1530
Russel P, Nurse P (1987) The mitotic inducer nim1+ functions in a regulatory network of protein kinase homologs controlling the initiation of mitosis. Cell 49:569–576
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Sehgal SN, Gibbons NE (1960) Effect of some metal ions on the growth of Halobacterium halobium. Can J Microbiol 6:165–169
Soret J, Krycève-Martinerie C, Crochet J, Perbal B (1985) Transformation of brown Leghorn chicken embryo fibroblasts by avian myeloblastosis virus proviral DNA. J Virol 55:193–205
Studzinski GP, Brelvi ZS, Feldman SC, Rosemary AW (1986) Participation of c-myc protein in DNA synthesis of human cells. Science 234:467–470
Woese CR, Fox GE (1977) Phylogenetic structure of the prokaryotic domain: the primary kingdoms. Proc Natl Acad Sci USA 74:5088–5090
Yamamoto K, Ikawa S, Akiyama T, Semba K, Nomura N, Miyajima N, Saito T, Toyoshima K (1986) Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor. Nature 319:230–234
Zillig W, Schnabel R, Stetter KO (1985) Origin of the eukaryotic cytoplasm. C.T. Microbiol Immunol 114:1–18
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Ben-Mahrez, K., Sougakoff, W., Nakayama, M. et al. Stimulation of an alpha like DNA polymerase by v-myc related protein of Halobacterium halobium . Arch. Microbiol. 149, 175–180 (1988). https://doi.org/10.1007/BF00422002
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DOI: https://doi.org/10.1007/BF00422002