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Unusual genetic phenomena associated with Tn5 mutagenesis in Alcaligenes eutrophus strain H1

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Abstract

Tn5 was introduced into Alcaligenes eutrophus strain H1 by a suicide vector pSUP1011. Physical characterization of mutants obtained after Tn5 mutagenesis revealed a relatively high frequency of plasmid curing, or deletion of a 50 kb plasmid DNA segment. Results of Southern hybridization and chromosomal walking indicate that the same continuous stretch of plasmid DNA (designated as D region of plasmid) is deleted in four independent isolates. Moreover, the same deletion of plasmid DNA is also observed in a mitomycin C-generated mutant strain H1-4.

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Author notes

  1. W.-Y. W. Chow

    Present address: Institute of Molecular Biology, Academica Sinica, Nanking, 115-29, Taipei, Republic of China

Authors and Affiliations

  1. Department of Genetics, Iowa State University, 50011, Ames, IA, USA

    W.-Y. W. Chow & A. G. Atherly

  2. Department of Botany, Iowa State University, 50011, Ames, IA, USA

    J. B. Peterson

Authors
  1. W.-Y. W. Chow
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  2. J. B. Peterson
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  3. A. G. Atherly
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Additional information

Journal Paper No. J-12095 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa. Project No. 2607, supported in part by a grant from the Iowa High Technology Council

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Chow, WY.W., Peterson, J.B. & Atherly, A.G. Unusual genetic phenomena associated with Tn5 mutagenesis in Alcaligenes eutrophus strain H1. Arch. Microbiol. 152, 289–295 (1989). https://doi.org/10.1007/BF00409665

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  • DOI: https://doi.org/10.1007/BF00409665

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