Abstract
The denitrifying bacterium, Achromobacter xylosoxidans NCIB 11015, was cultivated in meat extract-peptone medium and in Mn-free synthetic medium under denitrifying or non-denitrifying conditions. Electron paramagnetic resonance (EPR) spectra for the whole cells of the bacteria thus obtained were measured at 77K. The characteristic three-line signal was observed in the whole cells of the bacteria under denitrifying conditions, but not under non-denitrifying conditions. The three-line signal was more distinctly observed in the cells cultured in Mn-free medium. This signal could be assigned to nitrosylcytochrome c′ containing a five-coordinated nitrosylheme. The elemental composition in these cells is also discussed.
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Yoshimura, T., Shidara, S., Ozaki, T. et al. Five coordinated nitrosylhemoprotein in the whole cells of denitrifying bacterium, Achromobacter xylosoxidans NCIB 11015. Arch. Microbiol. 160, 498–500 (1993). https://doi.org/10.1007/BF00245312
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DOI: https://doi.org/10.1007/BF00245312