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Some effects of chloramphenicol on the metabolism of chlorella

I. The effect on protein, polysaccharide and nucleic acid synthesis

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Summary

Chloramphenicol at concentrations of 1–3 mg per ml does not show specific inhibition of protein synthesis in Chlorella. Thus, although incorporation of 14C-labelled phenylalanine, carbon dioxide and glucose into the protein fraction is inhibited by chloramphenicol, the antibiotic also prevents incorporation of 14C-carbon dioxide and 14C-glucose into polysaccharide, together with incorporation of 14C-adenine into RNA and DNA fractions.

The inhibitory effects are not specific for macromolecule biosynthesis since incorporation of 14C-carbon dioxide and 14C-phenylalanine into an alcohol-soluble fraction, and that of 14C-adenine into a cold acid-soluble fraction are also inhibited.

Incorporation of 14C-glucose into the alcohol-soluble fraction, and incorporation of 14C-uracil into the cold acid-soluble and RNA fractions are insensitive to the antibiotic. The respiration of 14C-glucose is inhibited by only 20% with a chloramphenicol concentration of 3 mg per ml.

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Authors and Affiliations

  1. Department of Botany, University College London, London, UK

    I. Morris

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  1. I. Morris
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Morris, I. Some effects of chloramphenicol on the metabolism of chlorella. Archiv. Mikrobiol. 54, 160–168 (1966). https://doi.org/10.1007/BF00408712

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  • DOI: https://doi.org/10.1007/BF00408712

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