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Verwertung von Cytosin und Uracil durch Hydrogenomonas facilis und Hydrogenomonas H 16

Utilisation of cytosine and uracil by Hydrogenomonas facilis and Hydrogenomonas H 16

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Summary

Hydrogenomonas facilis utilizes thymine, cytosine, and uracil as nitrogen sources; Hydrogenomonas H 16, however, only cytosine. Cytosine is completely metabolised by Hydrogenomonas facilis, but is only deaminated by Hydrogenomonas H 16 and the resulting uracil accumulates in the culture medium.

Cytosine deaminase activity was demonstrated in cell-free preparations from Hydrogenomonas H 16 which had been grown with cytosine as the sole nitrogen source. Enzyme activity was measured using a coupled enzyme assay in which glutamic acid dehydrogenase served as an accesory enzyme. An 18-fold increase in cytosine deaminase activity was observed in ammonia-grown cells during the incubation in a cytosine containing medium.

Zusammenfassung

Hydrogenomonas facilis verwertet Thymin, Cytosin und Uracil als N-Quelle, Hydrogenomonas H 16 dagegen nur Cytosin. Durch Hydrogenomonas facilis wird Cytosin vollständig abgebaut, durch Hydrogenomonas H 16 lediglich desaminiert, wobei das entstehende Uracil in der Nährlösung angehäuft wird.

In zellfreien Extrakten aus Hydrogenomonas H 16, die mit Cytosin als einziger N-Quelle gewachsen waren, wurde Cytosindesaminase-Aktivität im gekoppelten enzymatischen Test nachgewiesen, wobei Glutaminsäuredehydrogenase als Hilfsenzym diente. Mit Ammoniumchlorid herangezogene Zellen zeigten während der Indubation in Cytosin-haltigem Medium einen 18fachen Anstieg der spezifischen Cytosindesaminase-Aktivität.

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Authors and Affiliations

  1. Institut für Mikrobiologie der Universität Göttingen, Göttingen, Deutschland

    H. Kaltwasser & J. Krämer

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  1. H. Kaltwasser
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  2. J. Krämer
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Kaltwasser, H., Krämer, J. Verwertung von Cytosin und Uracil durch Hydrogenomonas facilis und Hydrogenomonas H 16 . Archiv. Mikrobiol. 60, 172–181 (1968). https://doi.org/10.1007/BF00407642

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  • DOI: https://doi.org/10.1007/BF00407642

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