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Apparent absence of relationship between repair of single strand breaks in DNA and gamma radiation resistance in Salmonella thompson

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Summary

0736 01

  1. 1.

    A mutant strain of Salmonella thompson (S. thompson/r), with an increased resistance to gamma radiation, did not appear to possess a DNA repair system, active in a “non-growth” medium, similar to that reported for Micrococcus radiodurans and Escherichia coli B/r.

  2. 2.

    DNA strand breaks occurred to a similar degree in both the sensitive parent strain and the resistant mutant strain during exposure to gamma radiation.

  3. 3.

    The DNA could be reconstituted in both strains, but only when the cells were incubated in a growth-promoting medium after irradiation.

  4. 4.

    Results indicated that inactivation of S. thompson by gamma radiation was not the result of the production of DNA single-strand breaks per se, and the resistance of the mutant strain, therefore, did not appear to be directly related to the ability to repair such breaks.

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References

  • Dean, C. J., Alexander, P.: Sensitisation of radio-resistant bacteria to X-rays by Iodoacetamide. Nature (Lond.) 196, 1324–1346 (1962).

    Google Scholar 

  • — Ormerod, M. G., Serianni, R. W., Alexander, P.: DNA strand breakage in cells irradiated with X-rays. Nature (London.) 222, 1042–1044 (1969).

    Google Scholar 

  • Erdman, I. E., Thatcher, F. S., MacQueen, K. F.: Studies on the irradiation of micro-organisms in relation to food preservation II. Canad. J. Microbiol. 7, 207–215 (1961).

    Google Scholar 

  • Freifelder, D.: Rate of production of single strand breaks in DNA by X-irradiation in situ. J. molec. Biol. 35, 303–309 (1968).

    Google Scholar 

  • Ginsberg, D. M., Webster, H. K.: Chemical protection against single-strand breaks in DNA of gamma irradiated E. coli. Radiat. Res. 39, 421–435 (1969).

    Google Scholar 

  • Haynes, R. H.: The interpretation of microbial inactivation and recovery phenomena. Radiat. Res. Suppl. 6, 1–29 (1966).

    Google Scholar 

  • McGrath, R. A., Williams, R. W.: Reconstruction in vivo of irradiated E. coli DNA; the rejoining of broken pieces. Nature (London.) 212, 534–535 (1966).

    Google Scholar 

  • Moseley, B. E. B., Laser, H.: Similarity of repair of ionizing and U. V. radiation damage in M. radiodurans. Nature (London.) 206, 373–375 (1965).

    Google Scholar 

  • Pontefract, R. D., Thatcher, F. S.: A cytological study of normal and radiation-resistant E. coli. Canad. J. Microbiol. 11, 271–278 (1965).

    Google Scholar 

  • Seefried, A. von: Studies on radiation resistance in S. thompson and S. typhimurium. Ph. D. thesis, University of Guelph (1968).

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Authors and Affiliations

  1. Department of Microbiology, University of Guelph, Guelph, Ontario, Canada

    M. C. Allwood & D. C. Jordan

  2. Department of Pharmacy, University of Nottingham, Nottingham, England

    M. C. Allwood

Authors
  1. M. C. Allwood
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  2. D. C. Jordan
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Allwood, M.C., Jordan, D.C. Apparent absence of relationship between repair of single strand breaks in DNA and gamma radiation resistance in Salmonella thompson . Archiv. Mikrobiol. 70, 161–166 (1970). https://doi.org/10.1007/BF00412207

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  • DOI: https://doi.org/10.1007/BF00412207

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