Summary
The development of various lactic acid bacteria during the early stages of fermentation (1–6 days after ensiling) in fish silage was studied. The first type of organisms that grew fastest was the oval cocci (most of them resembledLeuconostoc mesenteroides andStreptococcus lactis) followed by round cocci (mostlyS. faecalis). The number of oval cocci increased rapidly one day after ensiling and then decreased after 2–3 days. The round cocci increased first after 2–3 days and then decreased slowly after 4–5 days. Lactobacilli began to increase in number (more than 1010 per g silage) first after 6 days. Thus the pH in the silage was mainly lowered by the action of streptococci. Also in MRS medium the pH was more rapidly lowered byS. faecalis than byLactobacillus plantarum and other rods.
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References
Burkey, L. A., Kroulik, J. T., Bryant, M. P., Wiseman, H. G.: Bacterial activity in forage crop silage as indicated by the predominant groups or species of bacteria at different periods of storage. USDA, Agric. Res. Adm. (1953).
De Man, J. C., Rogosa, M., Sharpe, M. E.: A medium for the cultivation of lactobacilli. J. appl. Bact.23, 130–135 (1960).
Gibbs, B. M., Skinner, F. A.: Identification methods for microbiologists. Part A. London-New York: Academic Press 1966.
Kreuzer, R.: Untersuchungen zur Durchführung der Fischgärungssilage. II. Teil. Der Ablauf bakterieller und chemischer Vorgänge bei der Silierung. Arch. Fischereiwissenschaft,5, 47–58 (1954).
Langston, C. W., Bouma, C.: A study of the microorganisms from grass silage. II. The lactobacilli. Appl. Microbiol.8, 223–234 (1960a).
——: A study of the microorganisms from grass silage. I. The cocci. Appl Microbiol.8, 212–222 (1960b).
——: Types and sequence change of bacteria in orchardgrass and alfalfa silages. J. Dairy Sci.43, 1575–1583 (1960c).
—— Conner, R. M.: Chemical and bacteriological changes in grass silage during the early stages of fermentation. II. Bacteriological changes. J. Dairy Sci.45, 618–624 (1962).
—, Irvin, H., Gordon, C. H., Bouma, C., Wiseman, H. G., Melin, C. G., Moore, L. A.: Microbiology and chemistry of grass silage. USDA Tech. Bull.1187, 73 (1958).
Nilsson, R., Rydin, C.: The effect of malt enzymes on the biochemical changes occurring during ensilage. Proc. 8th. Internat. Grassland Congr., pp. 493–497 (1960).
Pedersen, C. S.: The effect of pure culture inoculation on the quality and chemical composition of sauerkraut. N. Y. St. Agric. exp. Sta. Tech. Bull.169, 22 (1930).
Steinböck, H.: Zur Frage des Stärkeabbauvermögens einiger Milchsäurebakterien. Milchwiss. Ber.12, 73–106 (1962).
Virtanen, A. I.: The microbiology of ensilage production. Zbl. Bakt. II. Abt.95, 472–477 (1936/37).
Wirahadikusumah, S.: PreventingClostridium botulinum type E poisoning and fat rancidity by silage fermentation. Lantbr. högsk. Ann.34, 551–689 (1968).
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Wirahadikusumah, S., Rajala, O., Lindgren, S. et al. Development of lactic acid bacteria during early stages of fermentation in fish silage. Archiv. Mikrobiol. 82, 95–100 (1972). https://doi.org/10.1007/BF01890400
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DOI: https://doi.org/10.1007/BF01890400