Skip to main content
Log in

Physiological studies on Phymatotrichum omnivorum

II. Physiocochemical properties of glycogen

  • Published:
Archiv für Mikrobiologie Aims and scope Submit manuscript

Summary

The physical and chemical properties and molecular structure of glycogen from Phymatotrichum omnivorum (Shear) Dugg. were studied. Glycogen samples from rabbit liver and shellfish were compared with Phymatotrichum glycogen in physicochemical properties. Analyses of purity showed the isolated glycogen was highly pure. Purified glycogen had an optical rotation of + 183.3°, and optical rotatory dispersion showed the absence of optically active substances between 200 and 600 nm. Maximum absorption of the glycogen-iodine complex was at 395 nm. I.R. spectra confirmed the α-1,4 linkage as well as α-d-glucopyranose units of the glycogen molecule. Alpha- and Beta-amylase studies indicated the average chain length was 13 glucose units with 43% limit dextrin. Results of periodate oxidation of glycogen agreed closely with the result of the amylase studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Betz, N. L., Nettles, W. C., Novak, A. F.: Physiocochemical characteristics of glycogen from Anthonomus grandis. Comp. Biochem. Physiol. 24, 163–175 (1968).

    PubMed  Google Scholar 

  • Carrol, N. V., Longley, R. W., Roe, J. H.: The determination of glycogen in liver and muscle by use of the anthrone reagent. J. biol. Chem. 220, 583–593 (1956).

    PubMed  Google Scholar 

  • Djerassi, C.: Optical rotatory disperison. New York: McGraw-Hill Book Co. 1960.

    Google Scholar 

  • Dunlap, A. A.: A convenient soil culture method for obtaining sclerotia of the cotton root rot fungus. Amer. J. Bot. 28, 945–947 (1941).

    Google Scholar 

  • Ergle, D. R.: The glycogen content of Phymatotrichum sclerotia. J. Amer. chem. Soc. 69, 2061–2062 (1947).

    Google Scholar 

  • Fales, F. W.: A reproducible periodate oxidation method for the determination of glycogen end-groups. Analyt. Chem. 31, 1898–1900 (1959).

    Google Scholar 

  • Folch, J. M., Lees, M., Sloane-Stanley, G. H.: A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem. 226, 497–509 (1957).

    PubMed  Google Scholar 

  • Harris, G., MacWilliam, I. C.: A dipping technique for revealing sugars on paper chromatograms. Chem. Int. (Lond.) 73, 249 (1954).

    Google Scholar 

  • Kjolberg, O. G., Manners, D. J., Wright, A.: α-1,4 glucosans. XVIII. The molecular structure of some glycogens. Comp. Biochem. Physiol. 8, 353–365 (1963).

    Google Scholar 

  • Manners, D. J.: The molecular structure of glycogens. Advanc. Carbohyd. Chem. 12, 261–298 (1957).

    Google Scholar 

  • Manners, D. J., Wright, A.: Determination of the average chain length of glycogens by α-amylolysis. J. chem. Soc. 1962, 1597–1602.

  • Moore, S., Stein, W. H.: A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J. biol. Chem. 211, 907–913 (1954).

    PubMed  Google Scholar 

  • Northcote, D. H.: The molecular structure and shape of yeast glycogen. Biochem. J. 53, 348–352 (1953).

    PubMed  Google Scholar 

  • Rogers, C. H.: Cotton root rot studies with special reference to sclerotia, cover crops, rotation tillage, seedling rates, soil fungicides and effects on seed quality. Tex. Agr. exp. Sta. Bull. 614 (1942).

  • Somogyi, M.: Notes on sugar determination. J. biol. Chem. 195, 19–23 (1952).

    Google Scholar 

  • Sumi, M.: Chemical constituents of spores of Aspergillus oryzae. Biochem. Z. 195, 161–174 (1928).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gunasekaran, M. Physiological studies on Phymatotrichum omnivorum . Archiv. Mikrobiol. 84, 69–76 (1972). https://doi.org/10.1007/BF00408083

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00408083

Keywords

Navigation