Abstract
The process of mating reaction of Saccharomyces cerevisiae was studied by electron microscopy. Prior to the dissolution of the part of the cell walls separating the conjugating pair of cells, the thinning of the electron transparent layer of the cell wall occurs at the part toward which the nuclei are migrating.
After the dissolution of the cell walls of the conjugating cells, the cell membranes become associated with each other, then to be broken and rejoined.
The first diploid bud emerges from about the middle of the zygote.
The morphological changes during the mating reaction are discussed in relation to the biochemical changes so far known.
Similar content being viewed by others
References
Conti, S. F., Brock, T. D.: Electron microscopy of cell fusion in conjugating Hansenula wingei. J. Bact. 90, 524–533 (1965)
Conti, S. F., Naylor, H. B.: Electron microscopy of ultrathin sections of Schizosaccharomyces octosporus. II. Morphological and cytological changes preceding ascospore formation. J. Bact. 79, 331–340 (1960)
Duntze, W., MacKey, V., Manney, T. R.: Saccharomyces cerevisiae: A diffusible sex factor. Science 168, 1472–1473 (1970)
Firnfaber, J., Windisch, S.: Über das Kopulationsverhalten von Saccharomyces-Hefen. Arch. Mikrobiol. 65, 329–345 (1969)
Herman, A. I.: Sex-specific growth responses in yeasts. Antonie van Leeuwenhoek 37, 379–384 (1971)
Hinkelmann, W., Kraepelin, G.: Kompartimentbildungen in Hungerzellen von Saccharomyces cerevisiae. Arch. Mikrobiol. 74, 258–272 (1970)
Osumi, M.: Electron microscopical observations on the formation of yeast-mitochondria. Bot. Mag. (Tokyo) 78, 231–239 (1965)
Sakai, K., Yanagishima, N.: Mating reaction in Saccharomyces cerevisiae. II. Hormonal regulation of agglutinability of a type cells. Arch. Mikrobiol. 84, 191–198 (1972)
Shimoda, C., Yanagishima, N.: Role of cell wall-degrading enzymes in auxin-induced cell expansion in yeast. Physiol. Plant. 24, 46–50 (1971)
Shimoda, C., Yanagishima, N.: Mating reaction in Saccharomyces cerevisiae. III. Changes in autolytic activity. Arch. Mikrobiol. 85, 310–318 (1972)
Shimoda, C., Yanagishima, N.: Mating reaction in Saccharomyces cerevisiae. IV. Retardation of deoxyribonucleic acid synthesis. Physiol. Plant. 29, 54–59 (1973)
Smith, D. G., Wilkie, D., Srivastava, K. C.: Ultrastructural changes in mitochondria of zygotes in Saccharomyces cerevisiae. Microbios 6, 231–238 (1972)
Yamaguchi, H., Kanda, Y., Iwata, K., Osumi, M.: Electron microscopical studies on the fungitoxicity of bisphenyl-(2-chlorophenyl)-1-imidazolyl methane (BAYb 5097): Preferential effect on the nuclear envelope in Candida albicans cells. J. Electron. Microscopy 22, 167–172 (1973)
Yanagishima, N.: Sexual hormones in yeast. Planta (Berl.) 87, 110–118 (1969)
Yanagishima, N.: Induced production of a sexual hormone in yeast. Physiol. Plant. 24, 260–263 (1971)
Yanagishima, N., Sakai, K., Shimoda, C.: Regulation of the mating reaction in Saccharomyces cerevisiae. Proc. IV IFS: Fermentation Technol. Today, pp. 853–856 (1972)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Osumi, M., Shimoda, C. & Yanagishima, N. Mating reaction in Saccharomyces cerevisiae . Arch. Microbiol. 97, 27–38 (1974). https://doi.org/10.1007/BF00403042
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00403042