Abstract
The effect of a single oral dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 10 μg · kg−1, on the status of vitamin A (retinol) was studied in rats during an eight week period after administration. Retinol was determined spectrophotometrically after separation by means of high pressure liquid chromatography.
In the liver of control animals the total storage and the concentration of retinol were found to increase linearly with time whilst in the TCDD-exposed animals both the concentration and total storage remained essentially unchanged. Differences in the storage levels were evident after 4 days, and after 8 weeks the treated animals had a total liver storage corresponding to about 30% of the controls. Retinol levels in serum were significantly higher in the treated animals after week 1 and 2.
Zusammenfassung
Der Einfluß einer oralen Einzeldosis von 2,3,7,8-Tetrachlordibenzo-p-dioxin (TCDD), 10 μg · kg−1, auf den Vitamin A (Retinol)-Statu wurde an Ratten 8 Wochen lang nach der Verabreichung untersucht. Das Retinol wurde spektrofotometrisch nach Trennung mit Hilfe von Hochdruckflüssigkeitschromatographie bestimmt.
In den Lebern der Kontrolltiere stieg die Totalspeicherung und Konzentration von Retinol linear mit der Zeit an, während in den TCDD exponierten Tieren sowohl die Konzentration als auch die Totalspeicherung im wesentlichen unverändert blieben. Unterschiede in der Speicherung waren schon nach 4 Tagen ersichtlich und nach 8 Wochen wiesen die exponierten Tiere nur noch 30% der totalen Leberspeicherung, verglichen mit der der Kontrollgruppe, auf. Der Retinolspiegel im Serum war in den exponierten Tieren ab Woche 2 und 3 signifikant höher.
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Thunberg, T., Ahlborg, U.G. & Johnsson, H. Vitamin A (retinol) status in the rat after a single oral dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin. Arch Toxicol 42, 265–274 (1979). https://doi.org/10.1007/BF00334840
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DOI: https://doi.org/10.1007/BF00334840
Key words
- Rats
- 2,3,7,8-tetrachlorodibenzo-p-dioxin
- Vitamin A
- Retinol
- Liver
- Serum
- Spectrophotometry
- High pressure liquid chromatography