Abstract
Two artificial antigens, N αNε-di(O, O-diisopropyl) phosphoryl l-lysine (DIP)-bovine serum albumin (BSA) conjugate (DIP-BSA) and DIP-KLH (keyhole limpet hemocyanin), were synthesized. Antibodies against sarin (O-isopropyl methylphosphonofluoridate) were obtained after immunization of rabbits with DIP-KLH conjugate. A competitive inhibition enzyme immunoassay (CIEIA) was developed to detect the organophosphorus nerve agent sarin. The antibody solutions could be inhibited by sarin as low as 10−6 mol/l, and the standard curve was linear over 3 orders of magnitude. The coefficients of intraassay and interassay variation of this method were 5.4–6.2% (n=11) and 8.0–9.5% (n=6) at a sarin concentration range of 10−3–10−6 mol/l, respectively. The recovery of sarin in water samples at the concentration of 5×10−5 mol/l was in the range of 96.8–102.5%. The specificity of the antiserum was assessed by comparing the inhibition induced by sarin with soman, Vx, isopropyl alcohol and isopropyl methyl phosphonic acid. The results showed that less than 5 mmol/l soman, 2 mmol/l Vx, 16 mmol/l isopropyl alcohol and 8 mmol/l isopropyl methyl phosphonic acid did not influence the determination of sarin in water samples.
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This work was supported by the National Nature Science Foundation and the State Education Commission of China
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Zhou, Yx., Yan, Qj., Ci, Yx. et al. Detection of the organophosphorus nerve agent sarin by a competitive inhibition enzyme immunoassay. Arch Toxicol 69, 644–648 (1995). https://doi.org/10.1007/s002040050226
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DOI: https://doi.org/10.1007/s002040050226