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Solid-phase-Radioimmunassay und Blutspiegel von Staphylokokken-α-Toxin

Solid-phase radioimmunassay and blood level of staphylococcal α-toxin

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Naunyn-Schmiedebergs Archiv für Pharmakologie und experimentelle Pathologie Aims and scope Submit manuscript

Summary

1. A solid-phase radioimmunassay has been developed which utilizes antibodies covalently bound to cellulose. Its advantages and reaction conditions have been demonstrated with staphylococcal α-toxin.

2. Sera of some rabbits, human plasma and especially native antitoxic sera contain precipitating antibodies which bind added radioactive toxin in proportion to the concentration of native toxin (“inverse” radioimmunassay).

3. Native and labelled toxins disappear very quickly from the circulating blood of rabbits (half-life time below 5 min). After its disappearance from the blood, radioactive toxin cannot be redistributed into the circulation by injection of antitoxin. Previous injection of normal or antitoxic bovine serum delays the elimination of α-toxin.

4. Previous ligation of both kidneys delays the elimination of labelled toxin from the blood stream considerably. Ligation of the ureters is less effective in this respect.

Zusammenfassung

1. Es wird ein „solid-phase-radioimmunassay“ unter Verwendung von kovalent an Cellulose gebundenen Antikörpern entwickelt. Vorteile und Reaktionsbedingungen werden am Beispiel des Staphylokokken-α-Toxins dargestellt.

2. Einige der untersuchten Kaninchenseren, Humanplasma und vor allem natives antitoxisches Serum enthalten präcipitierende Antikörper, welche von der zugesetzten Radioaktivität um so mehr binden, je höher die Konzentration an unmarkiertem Toxin ist („inverser“ Radioimmunassay).

3. Unmarkiertes und markiertes Toxin verschwinden ungewöhnlich schnell (Halbwertszeit < 5 min) aus dem zirkulierenden Blut des Kaninchens. Eliminiertes markiertes Toxin läßt sich durch Gabe von Antitoxin nicht in die Blutbahn zurückholen. Vorherige Applikation von Normal-Rinderserum und von antitoxischem Rinderserum verzögert die Elimination des α-Toxins.

4. Nach doppelseitiger Nierenligatur wird markiertes Toxin erheblich langsamer aus der Blutbahn eliminiert; Ligatur beider Ureteren ist in dieser Hinsicht weniger effektiv.

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Authors and Affiliations

  1. Pharmakologisches Institut der Justus Liebig-Universität Gießen, Germany

    E. Habermann, K. O. Räker & G. Zeuner

Authors
  1. E. Habermann
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  2. K. O. Räker
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  3. G. Zeuner
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Herrn Prof. Dr. Ludwig Lendle zum 70. Geburtstag gewidmet.

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Habermann, E., Räker, K.O. & Zeuner, G. Solid-phase-Radioimmunassay und Blutspiegel von Staphylokokken-α-Toxin. Naunyn-Schmiedebergs Arch. Pharmak. u. Exp. Path. 262, 165–182 (1969). https://doi.org/10.1007/BF00537657

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  • DOI: https://doi.org/10.1007/BF00537657

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