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Zur Frage der Bedeutung des Kininsystems beim thermischen Ödem der Rattenpfote

Investigations of the significance of the kinin system in the thermic edema of the bat paw

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Summary

  1. 1.

    The suboutis of rat paws heated (46,5° C) in situ has been perfused. Kinin activity could be demonstrated regularly in the fluid which was collected in ice. When the solutions were tested immediately after having passed the tissue, only some of the experiments yielded positive results. Native and125Jlabelled kininogen as well as kininogenase and kininase activities passed into the perfusates. The sensitivity to dextran and the kinin release on heating were, in contrast to recent reports, not correlated.

  2. 2.

    The release of the components of the kinin system approximately paralleled that of labelled human albumin. Their concentration rose until about 1 hour after the start of the heating. There was no priority of the components of the kinin system when compared with human albumin which can be regarded as permeability indicator.

  3. 3.

    Intravenously injected carboxypeptidase B, because of its lower molecular weight, entered the interstitial fluid more easily than did the plasma carboxypeptidase N. Its blood level decreased rapidly; but sufficient tissue concentrations could be maintained by intravenous infusions. Neither the volume nor the time dependence of the thermic edema changed during carboxypeptidase B-infusions. The same was true for infusions of trasylol, whereas phenylbutazone inhibited the edema significantly. Edema formed by short heating (30 sec, 55° C) was equally resistant to carboxypeptidase B.

  4. 4.

    In the skin and muscles of the heated rat paw, carbon particles mainly stained the capillary walls. This finding argues against a considerable involvement of “classical” mediators which should induce venular lesions.

  5. 5.

    Infusion of large amounts of bradykinin into the arterial supply did not imitate the thermic edema; neither has bradykinin been found in the perfusate of the subcutis.

  6. 6.

    In the light of these findings, a significant role of the kinin system in the thermic edema of the rat paw is to be doubted.

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Authors and Affiliations

  1. Pharmakologisches Institut der Justus Liebig-Universität Gießen, Deutschland

    D. Urbanitz, H. Wiegand & E. Habermann

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  1. D. Urbanitz
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  2. H. Wiegand
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  3. E. Habermann
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Additional information

Herrn Dr.Räker danken wir für die Herstellung und Messung der radioaktiven Substanzen, Herrn Dr.Török für die Anfertigung der histologischen Präparate. Der Deutschen Forschungsgemeinschaft verdanken wir eine Sachbeihilfe, den Farbenfabriken Bayer AG, Leverkusen, Carboxypeptidase B sowie Hippuryl-L-Arginin.

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Urbanitz, D., Wiegand, H. & Habermann, E. Zur Frage der Bedeutung des Kininsystems beim thermischen Ödem der Rattenpfote. Naunyn-Schmiedebergs Arch. Pharmak. 264, 476–493 (1969). https://doi.org/10.1007/BF01002384

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  • DOI: https://doi.org/10.1007/BF01002384

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