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Acetylcholine release from isolated vas deferens of the rat

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Summary

The release of acetylcholine was measured during field stimulation of the isolated vas deferens of the rat. The rate of release was frequency-dependent; the higher the frequency used, the higher was the amount of acetylcholine released. Tetrodotoxin reduced the release of acetylcholine induced by field stimulation, but pretreatment with 6-hydroxydopamine did not.

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References

  • Ambache, N., Zar, M. A.: Motor transmission in the vas deferens: The inhibitory action of noradrenaline. Brit. J. Pharmacol. 40, 556P-558P (1970).

    Google Scholar 

  • : Evidence against adrenergic motor transmission in the guinea-pig vas deferens. J. Physiol. (Lond.) 216, 359–389 (1971).

    Google Scholar 

  • Bentley, G. A., Smith, G.: Effect of alpha-adrenergic receptor blocking drugs on the response of vas deferens and arterial muscle to sympathetic drugs and stimulation. Circulat. Res. 21, Suppl. 3, 101–110 (1967).

    Google Scholar 

  • Birmingham, A. T.: The potentiation by anticholinesterase drugs of the responses of the guinea-pig isolated vas deferens to alternate preganglionic and postganglionic stimulation. Brit. J. Pharmacol. 27, 145–156 (1966).

    Google Scholar 

  • Wilson, B.: Preganglionic and postganglionic stimulation of the guinea-pig isolated vas deferens preparation. Brit. J. Pharmacol. 21, 569–580 (1963).

    Google Scholar 

  • Boyd, H., Chang, V., Rand, M. J.: The anticholinesterase activity of some antiadrenaline agents. Brit. J. Pharmacol. 15, 525–532 (1960).

    Google Scholar 

  • Burnstock, G., Holman, M. E.: An electrophysiological investigation of the action of some autonomic blocking drugs on transmission in the guinea-pig vas deferens. Brit. J. Pharmacol. 23, 600–612 (1964).

    Google Scholar 

  • Falck, B.: Observation on the possibilities of the cellular localisation of the monoamines by a fluorescent method. Acta physiol. scand. 56 Suppl., 197 (1962).

    Google Scholar 

  • Furness, J. B., Campbell, G. R., Gilland, S. M., Malmyoss, T., Cobb, J. L. S., Burnstock, G.: Cellular studies of sympathetic denervation produced by 6-Hydroxydopamine in the vas deferens. J. Pharmacol. exp. Ther. 174, 111–122 (1970).

    Google Scholar 

  • Huković, S.: Responses of the isolated sympathetic nerve-ductus deferens preparation of the guinea-pig. Brit. J. Pharmacol. 16, 188–194 (1961).

    Google Scholar 

  • Jacobowitz, D., Koelle, G. B.: Histochemical correlation of acetylcholinesterase and catecholamine in postganglionic autonomic nerves of the cat, rabbit and guinea-pig. J. Pharmacol. exp. Ther. 148, 225–237 (1965).

    Google Scholar 

  • Knoll, J., Vizi, E. S.: Presynaptic inhibition of acetylcholine release by endogenous and exogenous noradrenaline at high rate of stimulation. Brit. J. Pharmacol. 40, 554–555 P (1970).

    Google Scholar 

  • : Effect of frequency of stimulation on the inhibition by noradrenaline of the acetylcholine output from parasympathetic nerve terminals. Brit. J. Pharmacol. 41, 263–272 (1971).

    Google Scholar 

  • Kuriyama, H.: Electrophysiological observations on the motor innervation of the smooth muscle cells in the guinea-pig vas deferens. J. Physiol. (Lond.) 169, 213–228 (1963).

    Google Scholar 

  • Langley, J. N., Anderson, H. K.: The innervation of the pelvic and adjoining viscera. J. Physiol. (Lond.) 20, 372–406 (1896).

    Google Scholar 

  • McIntosh, F. C., Perry, W. L. M.: Biological estimation of acetylcholine. Methods in medical research, ed. R. W. Gerard, vol. 3. pp. 78–92. Chicago. The Year Book. Publ. Inc. 1950.

    Google Scholar 

  • Ohlin, P., Strömblad, B. C. R.: Observation on the isolated vas deferens. Brit. J. Pharmacol. 20, 299–306 (1963).

    Google Scholar 

  • Paton, W. D. M., Vizi, E. S.: The inhibitory action of noradrenaline on acetylcholine output by guinea-pig ileum longitudinal muscle strip. Brit. J. Pharmacol. 35, 10–28 (1969).

    Google Scholar 

  • , Zar, A.: The mechanism of acetylcholine release from parasympathetic nerves. J. Physiol. (Lond.) 215, 819–848 (1971).

    Google Scholar 

  • Robinson, P. M.: A cholinergic component in the innervation of the longitudinal smooth muscle of the guinea-pig vas deferens. J. Cell Biol. 41, 462–476 (1969).

    Google Scholar 

  • Sjöstrand, N. O.: The adrenergic innervation of the vas deferens and the accessory male genital glands. Acta physiol. scand. 65, Suppl. 257, 1–82 (1965).

    Google Scholar 

  • Vizi, E. S., Knoll, J.: The effect of sympathetic nerve stimulation and guanethidine on parasympathetic neuroeffector transmission; the inhibition of acetylcholine release. J. Pharm. Pharmacol. 23, 918–925 (1971).

    Google Scholar 

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Knoll, J., Somogyi, G.T., Illés, P. et al. Acetylcholine release from isolated vas deferens of the rat. Naunyn-Schmiedeberg's Arch. Pharmacol. 274, 198–202 (1972). https://doi.org/10.1007/BF00501855

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  • DOI: https://doi.org/10.1007/BF00501855

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