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The influence of blood flow on the absorption of 3-O-methylglucose from the jejunum of the rat

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Summary

  1. 1.

    Jejunal loops of anaesthetized rats were perfused (single pass perfusion and recirculation) with buffered solutions containing 3-O-methylglucose (3-OMG) in two concentrations: below (about 30 μM) and above (about 30 mM) blood glucose concentration. The blood flow, the appearance rate (in the intestinal venous blood) and the disappearance rate (from the intestinal lumen) were determined simultaneously, the accumulation in the gut wall at the end of the experiments.

  2. 2.

    At constant intermediate blood flow the appearance rate and disappearance rate decreased with time. A decrease of the blood flow rate from about 1.8 to about 0.3 ml min−1g−1 diminished the appearance and disappearance rate. An increase of blood flow from about 0.3 to about 1.9 ml min−1g−1 caused no increase of absorption; even a slight decrease was observed.

  3. 3.

    A kinetic analysis of the data showed that the different blood flow dependence of 3-OMG absorption in the experiments with increasing and decreasing blood flow can be explained sufficiently by a decrease of transport capacity with time, presumably due to an altered regional blood flow (decreased villous blood flow rate, unchanged total rate). A postischemic inhibition of 3-OMG absorption was of less importance.

  4. 4.

    The increase in perfusion rate from 0.1 (single pass perfusion) to 10 ml min−1 (recirculation) caused an increase of 3-OMG absorption and tissue accumulation.

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Authors and Affiliations

  1. Pharmakologisches Institut der Universität Tübingen, Germany

    B. Lichtenstein & D. Winne

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  1. B. Lichtenstein
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  2. D. Winne
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Supported by the Deutsche Forschungsgemeinschaft.

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Lichtenstein, B., Winne, D. The influence of blood flow on the absorption of 3-O-methylglucose from the jejunum of the rat. Naunyn-Schmiedeberg's Arch. Pharmacol. 279, 153–172 (1973). https://doi.org/10.1007/BF00503980

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