Summary
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1.
Jejunal loops of anaesthetized rats were perfused with hypotonic, isotonic and hypertonic buffered solutions containing 14C-labelled benzoic acid and salicylic acid at pH 6.2 and 2.2. The blood flow of the loop was maintained at an intermediate rate (0.78–0.97 ml min−1 g−1). The water net flux was determined by polyethylene glycol as non-absorbable marker and amounted up to +31 or-27 μl min−1 g−1.
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2.
A positive water net flux (leaving the gut lumen) increased the appearance rate of benzoic acid and salicylic acid in the intestinal venous blood by maximally 47 and 41%, a negative water net flux (entering the gut lumen) diminished the appearance rate by 28 and 37%.
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3.
The experimental data were analysed by means of a kinetic model. The parameters of the model were the epithelial permeability kF D , the serosal permeability k S F S and the sieving coefficient Φ=1−σ. The epithelial permeability was 0.115 and 0.107 for benzoic acid and salicylic acid at pH 6.2 and increased to 0.163 and 0.185 ml min−1 g−1 at pH 2.2. The serosal permeability was assumed to be identical for neutral and acidic pH and amounted to 0.046 and 0.112 ml min−1 g−1 indicating that a certain proportion of the absorbed drugs was transferred to the serosal side. The sieving coefficient was 2.92 and 2.31 at neutral pH and 3.79 and 3.56 at acidic pH.
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4.
The main resistance to the absorption of the two drugs is the epithelial membrane, since the effective blood flow and the unstirred layer are not ratelimiting. The high sieving coefficient for the two drugs is interpreted as indicating an interaction of drug and water molecules inside the lipid part of the cell membrane (which contains at least 30% water). The electric potential across the gut wall may contribute to the size of the sieving coefficient, if the drug molecules permeate also in the ionized form.
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5.
Using acidic perfusion solutions the epithelial permeability and the sieving coefficient were increased by the factor 1.3–1.5. This increase can be explained sufficiently by a facilitated entrance of the unionized drug molecules into the epithelial membrane.
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References
Barry, R. J. C., Dikstein, S., Matthews, J., Smyth, D. H.: Electrical potentials in the isolated intestine. J. Physiol. (Lond.) 155, 17 P-18 P (1961)
Barry, R. J. C., Matthews, J., Smyth, D. H., Wright, E. M.: Potential difference and intestinal transport of solutes and water. J. Physiol. (Lond.) 161, 17 P-18 P (1962)
Clarkson, T. W.: The transport of salt and water across isolated rat ileum. J. gen. Physiol. 50, 695–727 (1967)
Crone, H. D., Keen, T. E. B.: An in vitro study of the intestinal absorption of pyridinium aldoximes. Brit. J. Pharmacol. 35, 304–312 (1969)
Draper, N. R., Smith, H.: Applied regression analysis, 2nd ed. New York-London-Sidney: John Wiley and Sons, Inc. 1967
Finean, J. B.: Biophysical contributions to membrane structure. Quart. Rev. Biophys. 2, 1–23 (1969)
Forth, W., Furukawa, E., Leopold, G., Rummel, W.: Vergleichende Untersuchungen über die Resorption 3H-markeierter Herzglykoside. In: Radioisotope in Pharmakokinetik und klinischer Biochemie, pp. 123–128. G. Hoffmann u. H.-A. Ladner. Stuttgart-New York: Schattauer 1970
Hogben, C. A. M., Tocco, D. J., Brodie, B. B., Schanker, L. S.: On the mechanism of intestinal absorption of drugs. J. Pharmacol. exp. Ther. 125, 275–282 (1959)
Kedem, O., Katchalsky, A.: A physical interpretation of the phenomenological coefficients of membrane permeability. J. gen. Physiol. 45, 143–179 (1961)
Kedem, O., Katchalsky, A.: Permeability of composite membranes. Trans. Faraday Soc. 59, 1918–1953 (1963)
Kunze, H., Rehbock, G., Vogt, W.: Absorption of salicylic acid and its isomers from the rat jejunum. Naunyn-Schmiedegberg's Arch. Pharmacol. 273, 331–340 (1972)
Lifson, N., Gruman, L. M., Levitt, D. G.: Diffusive-convective models for intestinal absorption of D2O. Amer. J. Physiol. 215, 444–454 (1968)
Lifson, N., Hakim, A. A.: Simple diffusive-convective model for intestinal absorption of a nonelectrolyte (urea). Amer. J. Physiol. 211, 1137–1146 (1966)
Nogami, H., Matsuzawa, T.: Studies on absorption and excretion of drugs. I. Kinetics of penetration of acidic drug, salicylic acid, through the intestinal barrier in vitro. Chem. pharm. Bull. 9, 532–540 (1961)
Ochsenfahrt, H.: The mucosal-serosal transfer of drugs in the rat jejunum with and without blood flow. Naunyn-Schmiedebergs Arch. Pharmak. 270, Suppl. R 102 (1971)
Ochsenfahrt, H., Winne, D.: Der Einfluß der Durchblutung auf die Resorption von Arzneimitteln aus dem Jejunum der Ratte. Naunyn-Schmiedebergs Arch. Pharmak. 264, 55–75 (1969)
Ochsenfahrt, H., Winne, D.: Der Einfluß des Wassernettofluxes auf die Resorption von Arzneimitteln. Naunyn-Schmiedebergs Arch. Pharmak. 266, 414–415 (1970)
Ochsenfahrt, H., Winne, D.: The contribution of solvent drag to the intestinal absorption of tritiated water and urea from the jejunum of the rat. Naunyn-Schmiedeberg's Arch. Pharmacol. 279, 133–152 (1973)
Ochsenfahrt, H., Winne, D.: The contribution of solvent drag to the intestinal absorption of the basic drugs amidopyrine and antipyrine from the jejunum of the rat. Naunyn-Schmiedeberg's Arch. Pharmacol. 281, 175–196 (1974)
Schanker, L. S.: Physiological transport of drugs. Advanc. Drug Res. 1, 71–106 (1964)
Schanker, L. S., Tocco, D. J., Brodie, B. B., Hogben, C. A. M.: Absorption of drugs from the rat small intestine. J. Pharmacol. exp. Ther. 123, 81–88 (1958)
Smyth, D. H., Wright, E. M.: Streaming potentials in the rat small intestine. J. Physiol. (Lond.) 182, 591–602 (1966)
Staverman, A. J.: The theory of measurement of osmotic pressure. Rec. Trav. chim. Pays-Bas 70, 344–352 (1951)
Winne, D.: Die Pharmakokinetik der Resorption bei Perfusion einer Darmschlinge mit variabler Durchblutung. Naunyn-Schmiedebergs Arch. Pharmak. 268, 417–433 (1971)
Winne, D.: The influence of blood flow and water net flux on the absorption of tritiated water from the jejunum of the rat. Naunyn-Schmiedeberg's Arch. Pharmacol. 272, 417–436 (1972)
Winne, D., Remischovsky, J.: Der Einfluß der Durchblutung auf die Resorption von Harnstoff, Methanol und Äthanol aus dem Jenunum der Ratte. Naunyn-Schmiedebergs Arch. Phamak. 268, 392–416 (1971)
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Ochsenfahrt, H., Winne, D. The contribution of solvent drag to the intestinal absorption of the acidic drugs benzoic acid and salicylic acid from the jejunum of the rat. Naunyn-Schmiedeberg's Arch. Pharmacol. 281, 197–217 (1974). https://doi.org/10.1007/BF00503498
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DOI: https://doi.org/10.1007/BF00503498