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The influence of blood flow on the phlorizine-insensitive and sensitive galactose absorption in rat jejunum

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Summary

  1. 1.

    Jejunal loops of anaesthetized rats were perfused with buffered solutions (pH 6.8) containing d-galactose in two concentrations: below (100 μM) and above (100 mM) blood sugar concentration. The blood flow rate, the appearance rate (in the intestinal venous blood) and the disappearance rate (from the intestinal lumen) were determined simultaneously, the accumulation in gut tissue at the end of the experiments.

  2. 2.

    At constant intermediate blood flow the appearance rate and disappearance rate decreased with time. A decrease of the blood flow rate from about 1.9 to about 0.3 ml min−1 g−1 diminished both the appearance and the disappearance rate. An increase of blood flow from about 0.3 to about 1.9 ml min−1 g−1 did not increase the absorption rates; even a slight decrease was observed.

  3. 3.

    In the presence of phlorizine (2 mM) the absorption rates and the tissue accumulation of galactose is reduced. At constant intermediate blood flow the appearance and disappearance rate increased with time. A decrease of the blood flow rate from about 1.8 to about 0.3 ml min−1 g−1 diminished the appearance rate. An increase of the blood flow rate from about 0.3 to about 1.8 ml min−1 g−1 caused an increase of the appearance rate in contrast to the experiments without phlorizine. Therefore, the different blood flow dependence of the intestinal absorption observed with actively transported substances can be attributed to their non-passive component.

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References

  • Antonioli, J. A., Robinson, J. W. L., Vanotti, A.: Kinetics of the absorption of amino acids by the rat intestine in vivo. (in press)

  • Csáky, T. Z., Ho, P. M.: The effect of potassium on the intestinal transport of glucose. J. gen. Physiol. 50, 113–138 (1966a)

    Google Scholar 

  • Csáky, T. Z., Ho, P. M.: Effect of dimethylsufoxide on the intestinal sugar transport. Proc. Soc. exp. Biol. (N.Y.) 122, 860–865 (1966b)

    Google Scholar 

  • Esposito, B., Faelli, A., Capraro, V.: Sul meccanismo del transporte transepiteliale di aminoacidi e suoi rapporti col transporte contemporaneo di sodio e di glicoso in un preparato intestinale in vitro. Arch. Sci. biol. (Bologna) 48, 341–356 (1964)

    Google Scholar 

  • Förster, H., Menzel, B.: Zur Bestimmung der Michaelis-Konstanten für die intestinale Glucoseresorption bei Untersuchungen in vivo. Z. Ernährungsw. 11, 24–39 (1972)

    Google Scholar 

  • Guthrie, J. E., Quastel, J. H.: Absorption of sugars and amino acids from isolated surviving intestine after experimental shock. Arch. Biochem. 62, 485–496 (1956)

    Google Scholar 

  • Kimmich, G. A.: Preparation and properties of mucosal epithelial cells isolated from small intestine of the chicken. Biochemistry 9, 3659–3668 (1970)

    Google Scholar 

  • Lauterbach, F.: Untersuchungen zum Mechanismus der Resorption von 3-O-Methyl-Glucose an der isolierten Mucosa des Meerschweinchen-Dünndarms. Vortrag auf der 13. Frühjahrstagung der Deutschen Pharmakologischen Gesellschaft in Mainz, 21. März 1972

  • Levinson, R. A., Schedl, H. P.: Absorption of sodium, chloride, water, and simple sugars in rat small intestine. Amer. J. Physiol. 211, 939–942 (1966)

    Google Scholar 

  • Lichtenstein, B., Winne, D.: The influence of blood flow on the absorption of 3-O-methylglucose from the jejunum of the rat. Naunyn-Schmiedeberg's Arch. Pharmacol. 279, 153–172 (1973)

    Google Scholar 

  • Lluch, M.: Influencia del aporte de oxigeno en la absorción intestinal de glucosa. Rev. esp. Fisiol. 10, 275–314 (1954)

    Google Scholar 

  • McArdle, A. H., Bounous, G., Mavrias, D., Gurd, F. N.: Metabolism and function of the intestine following hemorrhagic shock. Surg. Forum 18, 7–9 (1967)

    Google Scholar 

  • Nelson, R. A., Beargie, R. J.: Relationship between sodium and glucose transport in canine jejunum. Amer. J. Physiol. 208, 375–379 (1965)

    Google Scholar 

  • Ochsenfahrt, H., Winne, D.: Der Einfluß der Durchblutung auf die Resorption von Arzneimitteln aus dem Jejunum der Ratte. Naunyn-Schmiedebergs Arch. Pharmak. 264, 55–75 (1969)

    Google Scholar 

  • Rider, A. C., Schedl, H. P., Nokes, G., Shining, S.: Small intestinal glucose transport. J. gen. Physiol. 50, 1173–1182 (1967)

    Google Scholar 

  • Robinson, J. W. L., Antonioli, V., Mirkovitch, V.: The intestinal response to ischemia. Naunyn-Schmiedebergs Arch. Pharmak. 255, 178–191 (1966)

    Google Scholar 

  • Robinson, J. W. L., Jéquier, J. C., Taminelli, F.: The measurement of amino acid absorption in vitro. Experimental studies and clinical perspectives. Gastroenterologia (Basel) 102, 292–299 (1964)

    Google Scholar 

  • Robinson, J. W. L., Mirkovitch, V.: The recovery of function and microcirculation in small intestinal loops following ischemia. Gut 13, 784–789 (1973)

    Google Scholar 

  • Roche, A. C., Bognel, J. C., Bognel, C., Bernier, J. J.: Correlation between the histological changes and glucose intestinal absorption following a single dose of 5-Fluorouracil. Digestion 3, 195–212 (1970)

    Google Scholar 

  • Schedl, H. P., Miller, D. L., Wilson, H. D., Flores, P.: α-Aminoisobutyric acid transport and tissue concentration at various intestinal sites. Amer. J. Physiol. 216, 1131–1138 (1969)

    Google Scholar 

  • Schedl, H. P., Wilson, H. D.: Effects of diabetes on intestinal growth and hexosetransport in the rat. Amer. J. Physiol. 220, 1739–1745 (1971)

    Google Scholar 

  • Wertheimer, E.: Phloridzinwirkung auf die Zuckerresorption. Pflügers Arch. ges. Physiol. 232, 515–528 (1933)

    Google Scholar 

  • Winne, D.: Der Einfluß der Durchblutung auf die Wasser- und Salzresorption im Jejunum der Ratte. Naunyn-Schmiedebergs Arch. Pharmak. 265, 425–441 (1970)

    Google Scholar 

  • Winne, D.: The influence of blood flow on the absorption of l- and d-phenylalanine from the jejunum of the rat. Naunyn-Schmiedeberg's Arch. Pharmacol. 277, 113–138 (1973)

    Google Scholar 

  • Winne, D., Remischovsky, J.: Intestinal blood flow and absorption of non-dissociable substances. J. Pharm. Pharmacol. 22, 640–641 (1970)

    Google Scholar 

  • Winne, D., Remischovsky, J.: Der Einfluß der Durchblutung auf die Resorption von Harnstoff, Methanol und Äthanol aus dem Jejunum der Ratte. Naunyn-Schmiedebergs Arch. Pharmak. 268, 392–416 (1971a)

    Google Scholar 

  • Winne, D., Remischovsky, J.: Der Einfluß der Durchblutung auf die Resorption von Polyalkoholen aus dem Jejunum der Ratte. Naunyn-Schmiedebergs Arch. Pharmak. 270, 22–40 (1971b)

    Google Scholar 

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  1. Pharmakologisches Institut der Universität Tübingen, Germany

    B. Lichtenstein & D. Winne

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  1. B. Lichtenstein
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  2. D. Winne
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Lichtenstein, B., Winne, D. The influence of blood flow on the phlorizine-insensitive and sensitive galactose absorption in rat jejunum. Naunyn-Schmiedeberg's Arch. Pharmacol. 282, 195–212 (1974). https://doi.org/10.1007/BF00499034

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