Summary
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1.
Noradrenaline release from the isolated rabbit heart was evoked by perfusion with a medium containing 135 mM potassium and 17 mM sodium ions (high K+-low Na+).
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2.
The noradrenaline output in response to high K+-low Na+ was dose-dependently decreased by methacholine (0.625–320 μM) and this effect was reserved by atropine 1.44 μM.
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3.
Lowering the calcium concentration of high K+-low Na+ from 1.8–0.1125 mM decreased the noradrenaline output by 85%. The effect of methacholine, expressed as % inhibition of noradrenaline release, was potentiated by lowering of the calcium concentration.
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4.
Both at normal and lowered calcium concentrations the inhibitory action of methacholine was larger from 0–5 than from 5–10 min after perfusion with high K+-low Na+.
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5.
Perfusion of hearts with media containing high K+-high Na+ or normal K+-low Na+ caused noradrenaline outputs somewhat smaller than those after high K+-low Na+. The release from 0–5 min was both calcium-dependent and inhibited by methacholine.
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6.
High K+ and/or low Na+ solutions caused an increase in coronary perfusion pressure which was little affected by the noradrenaline released simultaneously.
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7.
It is concluded that activation of muscarine receptors at the terminal adrenergic fibre decreases the availability of calcium for transmitter release.
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Dubey, M.P., Muscholl, E. & Pfeiffer, A. Muscarinic inhibition of potassium-induced noradrenaline release and its dependence on the calcium concentration. Naunyn-Schmiedeberg's Arch. Pharmacol. 291, 1–15 (1975). https://doi.org/10.1007/BF00510816
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DOI: https://doi.org/10.1007/BF00510816