Abstract
The effect of centrally administered norepinephrine (NE) into the lateral ventricle on choline acetylase activity (ChAc), endogenous levels of NE, dopamine (DA) and serotonin (5HT) was studied in different regions of the rat brain. In normal rats, the highest activity of choline acetylase was found in the cerebral cortex, followed by the brain stem, the diencephalon, the hypothalamus and least in the cerebellum. Chronic administration of NE significantly increased the choline acetylase activity in all regions studied, whereas both the acute and the in vitro studies showed no significant change except in the brain stem. Serotonin level was increased in the cerebellum, but decreased in the diencephalon and the brain stem. There was no significant alteration in the level of NE in all areas studied except in the hypothalamus where there was an increase in the mean concentration. Tissue level of DA showed a significant increase in the cerebral cortex and the hypothalamus. Behaviourally, there was a significant increase in food intake on the first day of treatment with no significant change in water intake. The data suggests that NE may be involved in the regulation of acetylcholine synthesis. The significance of interaction between different monoamines and acetylcholine may be important in the study of drug tolerance phenomena.
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References
Ansell, G. B., Beeson, M. F.: A rapid and sensitive procedure for the combined assay of noradrenaline, dopamine and serotonin in a single brain sample. Anal. Biochem. 23, 196 (1968).
Anton, A. H., Sayre, D. F.: A study of the factors affecting the aluminium oxide-trihydroxyindole procedure for the analysis of catecholamines. J. Pharmacol. 138, 360 (1962).
Blaschko, H.: Development of Current concepts of catecholamine formation. Pharmacol. Rev. 11, 307 (1959).
Bloom, F. E., Algeri, S., Groppetti, A., Revuelta, A., Costa, E.: Lesions of the central norepinephrine terminals with 6-OH-dopamine: Biochemistry and fine structure. Science 166, 1284 (1969).
Burn, J. H., Rand, M. J.: Sympathetic postganglionic cholinergic fibres. Brit. J. Pharmacol. 15, 56 (1960).
Chang, C. C.: A sensitive method for spectrofluorometric assay of catecholamines. Int. J. Neuropharma 3, 643 (1964).
Dempsey, P. J., Cooper, T.: Ventricular cholinergic receptor systems: Interaction with adrenergic systems. J. Pharmacol. exp. Ther. 167, 282 (1969).
Goldstein, M., Anagnoste, B., Owen, W. S., Battista, A. F.: The effects of ventromedial tegmental lesions on the disposition of dopamine in the caudate nucleus of the monkey. Brain Research 4, 151 (1965).
Ho, A. K. S., Freeman, S. E., Freeman, W. P., Lloyd, H. J.: Action of tricyclic antidepressant drugs on central processes involving acetylcholine. Biochem. Pharmacol. 15, 817 (1966).
- Loh, H. H.: Biochemical evidence of adrenergic interaction with cholinergic function in the central nervous system. II. The effect of dopamine and its analogues, 5-OH-dopamine and 6-OH-dopamine. Europ. J. Pharmacol. (submitted for publication).
— —, Craves, F., Hitzemann, R. J., Gershon, S.: The effect of prolonged lithium treatment on the synthesis rate and turnover of monoamines in the brain regions of rats. Europ. J. Pharmacol. 10, 72 (1970).
—, Paddle, B. M., Freeman, S. E.: The estimation of the activity of acetylcholinesterase and other esterases in the rat brain by an amperometric method. Biochem. Pharmacol. 14, 151 (1965).
Jocobowitz, D., Koelle, G. B.: Histochemical correlations of acetylcholinesterase and catecholamines in postganglionic autonomic nerves of the cat, rabbit and guinea pig. J. Pharmacol. exp. Ther. 148, 225 (1965).
Koelle, G. B.: A proposed dual neurohumoral role of acetylcholine; its functions at the pre- and post-synpatic sites. Nature (Lond.) 190, 208 (1961).
—: Cytological distributions and physiological functions of cholinesterases. Heffter-Huebner Handb. exp. Pharm., Suppl. 15, Berlin-Göttingen-Heidelberg: Springer 1963.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. J. biol. Chem. 193, 265 (1951).
McCaman, R. E., Hunt, J. M.: Microdetermination of choline acetylase in nervous tissue. J. Neurochem. 12, 253 (1965).
Schildkraut, J. J., Dodge, G. A., Logue, M. A.: Effects of tricyclic antidepressants on the uptake and metabolism of intracisternally administered norepinephrine-H3 in the rat brain. J. Psychiat. Res. 7, 29 (1969).
Singer, G. A., Ho, K. S., Gershon, S.: Changes in choline acetylase level in the central nervous system of the rat following intraventricular administration of nor-adrenaline. Nature (Lond.) (in press).
—, Montgomery, R. B.: Functional relationship of lateral hypothalamus and amygdala in control of drinking. Physiol. Behav. 4, 505 (1969).
Sjoqvist, F., Fredricsson, B.: Cholinesterase distribution in the sympathetic nervous system of the cat. Pharmacol. XX 8, 18 (1961).
Thoenen, H., Transer, J. P.: Chemical sympathectomy by selective destruction of adrenergic nerve endings with 6-hydroxydopamine. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 261, 271 (1968).
Uretsky, N. J., Iversen, L. L.: Effects of 6-hydroxydopamine on noradrenaline-containing neurones in the rat brain. Nature (Lond.) 221, 557 (1969).
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This work was supported by USPHS grant MH-12383; Dr. Ho is a recipient of a PMA Foundation grant award in morphology-pharmacology.
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Ho, A.K.S., Singer, G. & Gershon, S. Biochemical evidence of adrenergic interaction with cholinergic function in the central nervous system of the rat. Psychopharmacologia 21, 238–246 (1971). https://doi.org/10.1007/BF00403862
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DOI: https://doi.org/10.1007/BF00403862