Abstract
The topography of stereotyped behaviour produced by apomorphine in rats was studied by using either a scoring system, based on observation in a wire cage, or by quantification of horizontal and vertical activities, and of the total distances run in an open field, using an automatic recording system. The latter design was combined with a classification of the type of stereotyped behaviour observed during recording. In addition, the reproducibility of the nature of the stereotyped behaviour and its dose-dependence in individual animals was evaluated. In rats observed in a wire cage, apomorphine at lower doses (0.25 or 0.50 mg/kg SC) produced stereotyped sniffing. Increasing the doses led to stereotyped licking and the largest dose (5.00 mg/kg SC) produced predominantly stereotyped gnawing, as was demonstrated graphically. The type of behaviour produced by 2 mg/kg apomorphine in the open field was reproduced well in individuals after a second administration 4 days later. The shift from sniffing to gnawing was observed in most, but not all of the individually classified animals after administration of the largest dose (5 mg/kg). The locomotor part of motility was highest in “sniffing animals” and lower when gnawing occurred. The non-locomotor part of motility was low in “sniffing rats” and increased when licking and gnawing occurred. In some of the animals a characteristic “climbing” behaviour was observed in addition after the larger doses, which did not interfere with sniffing, licking or gnawing.
A combination of classification by observation and automatic recording seems the most appropriate way to study the topography of stereotyped behaviour produced by apomorphine.
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References
Amsler C (1923) Beiträge zur Pharmakologie des Gehirns. Arch Exper Pathol Pharmakol 97:1–14
Andén NE, Rubensson A, Fuxe K, Hökfelt T (1967) Evidence of dopamine receptor stimulation by apomorphine. J Pharm Pharmacol 19:627–629
Carlsson A (1975) Receptor-mediated control of dopamine metabolism. In: Usdin E, Bunney WE jr (eds) Pre- and postsynaptic receptors. Marcel Dekker Inc, New York, pp 49–63
Costall B, Naylor RJ (1973) The role of telencephalic dopaminergic systems in the mediation of apomorphine-stereotyped behaviour. Eur J Pharmacol 24:8–24
Costall B, Domeney AM, Naylor RJ (1982) Behavioural and biochemical consequences of persistent overstimulation of mesolimbic dopamine systems in the rat. Neuropharmacology 21:327–335
Ernst AM (1967) Mode of action of apomorphine and dexamphetamine in gnawing compulsions in rats. Psychopharmacology 10:316–323
Fray PJ, Sahakian BJ, Robbins TW, Koob GF, Iversen SD (1980) An observational method for quantifying the behavioral effects of dopamine agonists: contrasting effects of d-amphetamine and apomorphine. Psychopharmacology 69:253–259
Gordon D, Beck CHM (1984) Subacute apomorphine injections in rats: effects on components of behavioral stereotypy. Behav Neural Biol 41:200–208
Harnack E (1974) Über die Wirkung des Apomorphins am Säugetier und am Frosch. Arch Exper Pathol Pharmakol 2:254–306
Joyce E, Iversen SD (1984) Dissociable effects of 6-OHDA-induced lesions of neostriatum on anorexia, locomotor activity and stereotypy: the role of behavioural competition. Psychopharmacology 83:363–366
Kelly PH, Seviour PW, Iversen SD (1975) Amphetamine and apomorphine responses in the rat following 6-OHDA lesions of the nucleus accumbens septi and corpus striatum. Brain Res 94:507–522
Lewis MH, Baumeister AA, McCorkle DL, Mailman RB (1985) A computer-supported method for analyzing behavioral observation: studies with stereotypy. Psychopharmacology 85:204–209
Ljungberg T, Ungerstedt U (1977) Different behavioural patterns induced by apomorphine: evidence that the method of administration determines the behavioural response to the drug. Eur J Pharmacol 46:41–50
Maj J, Grabowska M, Gajda L (1972) Effect of apomorphine on motility in rats. Eur J Pharmacol 17:208–214
Mogilnicka E, Klimek V (1977) Drugs affecting dopamine neurons and yawning behaviour. Pharmacol Biochem Behav 7:303–305
Robbins TW (1977) A critique of the methods available for the measurements of spontaneous motor activity. In: Iversen LL, Iversen SD, Snyder SH (eds) Handbook of Psychopharmacology, Vol 7: Principles of behavioral pharmacology. Plenum Press, New York London, pp 37–82
Strömbom U (1976) Catecholamine receptor agonists: effects on motor activity and rate of tyrosine hydroxylation in mouse brain. Naunyn-Schmiedeberg's Arch Pharmacol 292:167–176
Ungerstedt U (1979) Central dopamine mechanisms and unconditioned behaviour. In: Horn AS, Korf J, Westerink BHC (eds) The neurobiology of dopamine. Academic Press, London New York San Francisco, pp 577–596
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Havemann, U., Magnus, B., Möller, H.G. et al. Individual and morphological differences in the behavioural response to apomorphine in rats. Psychopharmacology 90, 40–48 (1986). https://doi.org/10.1007/BF00172869
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DOI: https://doi.org/10.1007/BF00172869