Summary
Intraluminal flow velocities and tubular dimensions were estimated for superficial and deep proximal tubules of non-diuretic kidneys with a technique developed byHanssen. Kidneys of anesthetized rats were frozen in vivo 4–20 sec after an i.v. injection of 0.05 ml 10–20% Na ferrocyanide. Ferrocyanide was located as prussian blue in microdissected tubules after freeze-substitution and maceration of the kidneys. Its position was used to indicate the movement of glomerular filtrate along the tubule. Ferrocyanide clearance was slightly less than that of inulin at low plasma ferrocyanide levels. They were equal at higher plasma levels. The two clearances were sufficiently alike, however, to justify using ferrocyanide as an indicator of glomerular filtrate in these experiments. Transient small decreases in systemic blood pressure and increases in renal blood flow followed i.v. injections of 0.05 ml ferrocyanide and 0.05 ml 5% lissamine green. There was no evidence of continuing intraluminal flow when the kidney was rendered ischemic by ligating the renal pedicle. The mean distance between glomerulus and prussian blue front in kidneys frozen at different intervals after a ferrocyanide injection increased curvilinearly with time in a manner compatible with its indicating mean flow velocity. The distance between glomerulus and dye front in tubules from any one kidney was proportional to tubular length. Flow velocities were similar when measured with lissamine green in vivo or with ferrocyanide in microdissected tubules. Velocities decreased whenC inulin was reduced by partial compression of the renal artery. The reduction was greater in superficial than in deep tubules suggesting that flow velocities vary non-uniformly as gfr changes. Luminal diameters measured in microdissected tubules were similar to other estimates of in vivo tubular dimensions. There was no apparent difference between the length or luminal diameter of proximal tubules from the surface and deeper portions of the cortex in these rats.
Similar content being viewed by others
References
Baines, A. D., P. P. Leyssac, andC. W. Gottschalk: Proximal luminal volume and fluid reabsorption in the rat kidney. Acta physiol. scand.74, 440 (1969).
—: Functional heterogeneity of nephrons. II. Filtration rates, intraluminal flow velocities and fractional water reabsorption. Pflügers Arch.308, 260 (1969).
Barger, A. C.: Renal hemodynamic factors in congestive heart failure. Ann. N. Y. Acad. Sci.139, 276 (1966).
Brandis, M., G. Braun-Schubert, andK. H. Gertz: Retrograder Flüssigkeitstrom in Kortikale Tubuli bei Aortenabklemmung. V. Symp. Gesellschaf. f. Nephrologie, September 1967, Lausanne.
Bray, G. A.: A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analyt. Biochem.1, 279 (1960).
Burg, M. B., L. Isacson, J. Grantham, andJ. Orloff: Electrical properties of isolated perfused rabbit renal tubules. Amer. J. Physiol.215, 788 (1968).
Caro, C. G.: The dispersion of indicator flowing through simplified models of the circulation and its relevance to velocity profile in blood vessels. J. Physiol. (Lond.)185, 501 (1966).
Friedlander, S. K., andM. Walser: Some aspects of flow and diffusion in the proximal tubule of the kidney. J. theoret. Biol.8, 87 (1965).
Gertz, K. H., J. A. Mangos, G. Braun, andH. D. Pagel: On the glomerular tubular balance in the rat kidney. Pflügers Arch. ges. Physiol.285, 360 (1965).
Hanssen, O. E.: A histochemical method for evaluation of excreted sodium ferrocyanide in isolated tubules of the mouse kidney. Acta path. microbiol. scand.44, 363 (1958).
—: Early post mortem renal changes studied in mice with one kidney exteriorized. I. The reliability of the experimental method as evaluated by the diuretic response to mannitol and sodium ferrocyanide. Acta path. microbiol. scand.49, 208 (1960).
—: The relationship between glomerular filtration and length of the proximal convoluted tubules in mice. Acta path. microbiol. scand.53, 265 (1961).
Hanssen, O. E.: Method for comparison of glomerular filtration in individual rat nephrons. 2nd Int. Cong. Nephrol. Prague, 527 (1963).
Leyssac, P. P.: Some characteristics of the proximal tubular wall related to reabsorption during luminal occlusion following interruption of glomerular filtration. Acta physiol. scand.63, 36 (1965).
Maunsbach, A. B.: Observations on the segmentation of the proximal tubule in the rat kidney. J. Ultrastruct. Res.16, 239 (1966).
Munnell, E. R., andD. E. Gregg: A small adjustable renal artery clamp for production of chronic hypertension in the rat. Proc. Soc. exp. Biol. (N. Y.)73, 645 (1950).
Oliver, J., andM. MacDowell: The structural and functional aspects of the handling of glucose by the nephrons and the kidney and their correlation by means of structural-functional equivalents. J. clin. Invest.40, 1093 (1961).
Rector, F. C., Jr., E. P. Brunner, andD. W. Seldin: Mechanism of glomerulo tubular balance. I. Effect of aortic constriction and elevated uretero pelvic pressure on glomerular filtration rate, fractional reabsorption, transit time and tubular size in the proximal tubule of the rat. J. clin. Invest.45, 590 (1966).
Steinhausen, M., A. Loreth u.D. Olson: Harnstromes, seine Beziehungen zum Blutdruck und zur Inulin-clearance. Pflügers Arch. ges. Physiol.286, 118 (1965).
Taylor, G.: Dispersion of soluble matter in solvent flowing slowly through a tube. Proc. roy. Soc. (Lond.) Ser. A219, 186 (1953).
Author information
Authors and Affiliations
Additional information
Supported in part by U.S.P.H.S.Grant HE-02334
Rights and permissions
About this article
Cite this article
Baines, A.D., Baines, C.J. & de Rouffignac, C. Functional heterogeneity of nephrons. Pflugers Arch. 308, 244–259 (1969). https://doi.org/10.1007/BF00586557
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00586557
Key-Words
- Flow Velocity in Single Nephron
- Ferrocyanide Clearance
- Ferrocyanide Located in Microdissected Nephrons
- Non-Uniformity of Nephron Function
- Nephron Dimensions