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Étude chez le rat des variations du débit individuel de filtration glomérulaire des néphrons superficiels et profonds en fonction de l'apport sodé

Variations in glomerular filtration rate of single superficial and deep nephrons under various conditions of sodium intake in the rat

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Summary

The intrarenal distribution of single glomerular filtration rates (SGFR) was studied in rats previously fed a normal or a low sodium diet during antidiuresis, or in rats previously fed a high sodium diet during hypertonic saline diuresis. SGFR were determined either indirectly by Hanssen's technique, or directly by means of a modification of this technique which gives absolute values [23].

In the normal rats, the mean value of SGFR of superficial and juxtamedullary nephrons was equal to 27.9 nl/min and 38.5 nl/min, respectively. There was no appreciable difference between SGFR in our normal and salt-deprived rats. In the salt-loaded rats, the mean value of SGFR of superficial and juxtamedullary nephrons reached 52.4 nl/min and 52.7 nl/min, respectively.

Therefore, the increase of the GFR of the whole kidney during salt loading was due to an increase of SGFR of all the nephrons. However, the increase of SGFR was more important for the superficial ones and the ratio SGFR superficial nephrons/SGFR juxtamedullary nephrons which was 0.7 in the normal rats reached 1.0 in the salt-loaded rats, indicating that there was no more functional heterogeneity of nephrons during our salt loadings. The variation of SGFR is discussed in relation to the sodium dependent variation of the intrarenal distribution of renin observed by many authors.

Résumé

La distribution intrarénale des débits de filtration glomérulaire par néphron (fg) est étudiée en fonction de l'apport sodé chez trois groupes de rats: normaux, carencés en sodium et surchargés en sodium. Les fg ont été déterminées soit indirectement par la méthode de Hanssen soit grâce à une modification de cette méthode qui procure directement les valeurs absolues [23].

Chez les rats normaux, la fg est égale à 27,9 nl/min pour les néphrons superficiels et à 38,5 nl/min pour les néphrons juxtamédullaires. Il n'existe pas de différence décelable entre les fg de nos rats sans sel et celles de nos rats normaux. Chez les rats surchargés en sel, les fg des néphrons superficiels et juxtamédullaires passent respectivement à 52,4 nl/min et à 52,7 nl/min.

Au cours de nos surcharges sodées l'augmentation de filtration glomérulaire du rein résulte donc d'une augmentation de la fg de tous les néphrons. Celle-ci est cependant nettement plus marquée pour les néphrons superficiels et le rapport fg néphrons superficiels/fg néphrons juxtamédullaires qui est de 0,7 environ chez les rats normaux et sans sel, atteint 1,0 au cours de nos surcharges sodées. Ainsi dans cette dernière condition physiologique l'hétérogénéité fonctionnelle des néphrons disparaît. La variation des fg est discutée en fonction de ce que l'on sait de la variation de la distribution intrarénale de la rénine sous l'influence de l'apport sodé.

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Authors and Affiliations

  1. Département de Biologie, C. E. N. Saclay, B. P. no2, F-91, Gif-sur-Yvette, (France)

    C. de Rouffignac, J. P. Bonvalet (Docteur en Médecine, Attaché de Recherche à l'Institut National de la Santé et de la Recherche Médicale) & S. Deiss

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  1. C. de Rouffignac
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  2. J. P. Bonvalet
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  3. S. Deiss
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Additional information

Ce travail a été, en partie, présenté au 4ème Congrès International de Néphrologie, Stockholm 1969.

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de Rouffignac, C., Bonvalet, J.P. & Deiss, S. Étude chez le rat des variations du débit individuel de filtration glomérulaire des néphrons superficiels et profonds en fonction de l'apport sodé. Pflugers Arch. 317, 141–156 (1970). https://doi.org/10.1007/BF00592498

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