Summary
A simple method using glass capillaries instead of microcuvettes for measurement of inulin in nanoliter samples is given. Inulin was determined with anthron reagent (5 or 10 nl samples +3 μl anthron reagent). Glass capillary tubes (o.d.=1 mm, i.d.=0.68 mm, length=150 mm) in which the chemical reaction took place during incubation at 56°C were directly introduced into the optical system of a Zeiss spectrophotometer PMQ II with sphere attachment and objective.
Extinction was measured vertically to the axis of the capillary. The changes of extinction of 20 different capillaries with the blank at different positions was only 1.13×10−3. The exactness of measurement in the concentration range of 100 ≤ 200 ≥ 400 ≥ 750 ≥ 1500 ≥ 3000 mg-% inulin was for 5nl/3 μl: 19.8 ≥ 11.0 ≥ 6.7 ≥ 4.7 ≥ 3.0 2.2%. 10nl/3 μl: 13.0 ≥ 8.4 ≥ 5.1 ≥ 3.9%.
This method of measurement may also be applicable for other colorimetric reactions with nanoliter samples.
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This work was supported by “Fonds zur Förderung der wissenschaftlichen Forschung”.
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Holzer, F., Hohenegger, M. & Kink, H. A simple method for measurement of inulin in nanoliter samples using glass capillaries. Pflugers Arch. 343, 179–183 (1973). https://doi.org/10.1007/BF00585714
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DOI: https://doi.org/10.1007/BF00585714