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Single Na channels in mouse neuroblastoma cell membrane

Indications for two open states

  • Excitable Tissues and Central Nervous Physiology
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Abstract

Single sodium channel currents were studied in mouse neuroblastoma cells. Channel conductance (γ) was 11.6±3.4 pS at 6–8°C. Unitary current amplitudes and channel activity increased with increasing temperature. The Arrhenius plot of the conductance was linear between 5 and 35°C with an activation enthalpy of 27.1 kJ/mole (Q 10 =1.28). Amplitude distributions were fitted by the sum of two Gaussian functions indicating the presence of two different single channel amplitudes: smalleri 1 and largeri 2. The relative probability of appearance ofi 1, which has a shorter mean open time, was higher during the early phase of depolarization (t<16 ms). The open time histograms, inactivating phase of macroscopic currents and delay time histograms were fitted by the sum of two exponentials. The distinct kinetic and steady-state parameters reflect two open states of sodium channels.

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Author notes

  1. Tibor Kiss

    Present address: Balaton Limnological Research Institute of the Hungarian Academy of Sciences, H-8237, Tihany, Hungary

Authors and Affiliations

  1. I. Physiologisches Institut der Universität des Saarlandes, D-6650, Homburg/Saar, Federal Republic of Germany

    Karoly Nagy, Tibor Kiss & Detlef Hof

Authors
  1. Karoly Nagy
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  2. Tibor Kiss
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  3. Detlef Hof
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Nagy, K., Kiss, T. & Hof, D. Single Na channels in mouse neuroblastoma cell membrane. Pflugers Arch. 399, 302–308 (1983). https://doi.org/10.1007/BF00652757

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  • DOI: https://doi.org/10.1007/BF00652757

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