Abstract
The effects of glucagon on water and electrolyte transport in the kidney were investigated on hormone-deprived rats, i.e. thyroparathyroidectomized diabetes insipidus Brattleboro rats infused with somatostatin. Glucagon consistently inhibited the reabsorption of water and Na+, Cl−, K+ and Ca2+ along the proximal tubule accessible to micropuncture, leaving the reabsorption of inorganic phosphate (Pi) untouched. In the loop, besides its previously described stimulatory effects on Na+, Cl−, K+, Ca2+ and Mg2+ reabsorption, glucagon strongly inhibited Pi reabsorption, very probably in the proximal straight tubule. These effects resulted in a significant phosphaturia and considerable reductions of Mg2+ and Ca2+ excretions. The effects of glucagon at both the whole kidney and the nephron levels are very similar to those previously described for calcitonin. In the absence of an adenylate cyclase system sensitive to glucagon and calcitonin in the rat proximal tubule, and from the analogy of their physiological effects with those elicited by parathyroid hormone, it is suggested that glucagon and calcitonin exert their inhibitory effects on Na and Pi reabsorption in the proximal tubule through another pathway, which could be the phosphoinositide regulatory cascade.
Similar content being viewed by others
References
Agus ZS, Puschett JB, Senesky D, Goldberg M (1971) Mode of action of parathyroid hormone and cyclic adenosine 3′,5′ monophosphate on renal tubular phosphate reabsorption in the dog. J Clin Invest 50:617–626
Alpern RJ (1990) Cell mechanisms of proximal tubule acidification. Physiol Rev 70:79–114
Bailly C, Roinel N, Amiel C (1984) PTH-like glucagon stimulation of Ca and Mg reabsorption in Henle's loop of the rat. Am J Physiol 246:F205-F212
Chen PS, Toribara TY, Warner H (1956) Microdetermination of phosphorus. Anal Chem 28:1756–1758
Dennis VW, Bello-Reuss E, Robinson RR (1977) Response of phosphate transport to parathyroid hormone in segments of rabbit nephron. Am J Physiol 233:29–39
Di Stefano A, Wittner M, Nitschke R, Braitsch R, Greger R, Bailly C, Amiel C, Elalouf JM, Roinel N, de Rouffignac C (1989) Effects of glucagon on Na+, Cl−, K+, Mg2+, and Ca2+, transports in cortical and medullary thick ascending limbs of mouse kidney. Pflügers Arch 414:640–646
Elalouf JM, Roinel N, de Rouffignac C (1984) ADH-like effects of calcitonin on electrolyte transport by Henle's loop of rat kidney. Am J Physiol 246:F213-F220
Emmanouel DS, Jaspan JB, Rubinstein AH, Huen AHJ, Fink E, Katz AI (1978) Glucagon metabolism in the rat. Contribution of the kidney to the metabolic clearance of the hormone. J Clin Invest 62:6–13
Gekle D (1971) Der Einfluß von Parathormon auf die Nierenfunktion. Pflügers Arch 323:96–120
Gmaj P, Murer H (1986) Cellular mechanisms of inorganic phosphate transport in kidney. Physiol Rev 66:36–70
Helme-Colb C, Montrose MH, Murer H (1990) Parathyroid hormone regulation of Na+/H+ exchange in opposum kidney cells: polarity on mechanisms. Pflügers Arch 416:615–623
Helme-Colb C, Montrose MH, Stange G, Murer H (1990) Regulation of Na+/H+ exchange in Oppossum kidney cells by parathyroid hormone, cyclic AMP and phorbol esters. Pflügers Arch 415:461–470
Morel F, Doucet A (1986) Hormonal control of kidney functions at the cell level. Physiol Rev 66:377–468
Morel F, Roinel N (1969) Application de la microsonde électronique à l'analyse élémentaire quantitative d'échantillons biologiques liquides d'un volume inférieur à 10−91. J Chim Phys-Chim Biol 66:1084–1091
Poujeol P, Touvay C, Roinel N, de Rouffignac C (1980) Stimulation of renal magnesium reabsorption by calcitonin in the rat. Am J Physiol 239:F524-F532
Quamme G, Pfeilschifter J, Murer H (1989) Parathyroid hormone inhibition of Na+/phosphate cotransport in OK cells: requirement of protein kinase C-dependent pathways. Biochim Biophys Acta 1013:159–165
Quamme G, Pfeilschifter J, Murer H (1989) Parathyroid hormone inhibition of Na+/phosphate cotransport in OK cells: intracellular [Ca2+] as second messenger. Biochim Biophys Acta 103:166–172
Reshkin SJ, Forgo J, Murer H (1990) Functional asymmetry in phosphate transport and its regulation in opposum kidney cells: parathyroid hormone inhibition. Pflügers Arch 416:624–631
Rocha A, Magaldi J, Kokko J (1977) Calcium and phosphate transport in isolated segments of rabbit Henle's loop. J Clin Invest 59:975–983
Roobol A, Alleyne GA (1973) Regulation of renal gluconeogenesis by calcium ions, hormones and adenosine 3′:5′-cyclic monophosphate. Biochem J 134:157–165
de Rouffignac C (1991) Regulation of magnesium excretion. In: Coe FL, Favus MJ (eds) Disorders of bone and mineral metabolism. Raven, New York (in press)
de Rouffignac C, Di Stefano A, Wittner M., Roinel N, Elalouf JM (1991) Consequences of the differential effects of ADH and other peptide hormones on the thick ascending limb of the mammalian kidney. Am J Physiol 260:R1023-R1035
Suzuki M, Kawaguchi Y, Kurihara S, Miyahara T (1989) Heterogeneous response of cytoplasmic free Ca2+ in proximal convoluted and straight tubule cells in primary cultures. Am J Physiol 257:F724-F731
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
de Rouffignac, C., Elalouf, J.M. & Roinel, N. Glucagon inhibits water and NaCl transports in the proximal convoluted tubule of the rat kidney. Pflügers Archiv 419, 472–477 (1991). https://doi.org/10.1007/BF00370791
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00370791