Abstract
Regulatory volume decrease after exposure to hypo-osmotic media in MDCK epithelial cells results from activation of both K+ and Cl− conductances. Swelling-stimulated 86Rb(K) losses were observed only across the basal-lateral membrane and were relatively insensitive to 10 mM Ba2+. The effect of hypo-osmotic media upon MDCK epithelia mounted in Ussing chambers has been investigated. Exposure of the basal-lateral surfaces to hypo-osmotic media resulted in a transient stimulation of inward short-circuit current (I sc) followed by inhibition of inward I sc in both control layers and in layers where inward current (due to transepithelial Cl− secretion) was first stimulated by 5 μM prostaglandin E1 (PGE1). The transient stimulation of inward current by hypo-osmotic media was not markedly attenuated by 10 mM Ba2+ in PGE1-stimulated layers. After stimulation of inward (Cl−-secretory) current to high levels by 10μM adrenaline, the predominant effect of basal-lateral exposure to hypo-osmotic media was an inhibition of the inward current. This inhibition was partially reversed by 40μM 4,4′-diisothiocyanatostilbene-2,2′-disulphonate (DIDS). The stimulation, then inhibition, of inward I sc is likely to be the result of separate swelling-induced K+ and Cl− conductances (respectively) at the basal-lateral membrane. The swelling-stimulated Cl− conductance is distinct from the apical Cl− conductance regulated by PGE1 or adrenaline.
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Simmons, N.L. The effect of hypo-osmolarity upon transepithelial ion transport in cultured renal epithelial layers (MDCK). Pflügers Arch 419, 572–578 (1991). https://doi.org/10.1007/BF00370297
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DOI: https://doi.org/10.1007/BF00370297