Abstract
The temperature-sensitive mutant cells (tsFT101) derived from a mouse mammary carcinoma cell line, FM3A, become multinucleated at a non-permissive temperature of 39°C. To further understand the molecular mechanism of such cytokinetic disturbance, we examined the expression of profilin, the main regulator of the transition of globular actin (G-actin) to filamentous actin (F-actin). RT-PCR analysis of mouse profilin cDNA from tsFT101 showed a point mutation (177 A → G) which was a wobble mutation causing no change in the encoded amino acid. The expression level of profilin mRNA was, however, diminished in cultured tsFT101 cells under non-permissive temperatures compared with wild-type FM3A cells in association with multinucleation. A stable transfection of profilin cDNA expression vector to tsFT101 cells prevented multinuclear cell formation when cultured at 39°C. In contrast, antisense profilin cDNA expression vector did not alter multinuclear cell formation. The primary cause of the cytokinetic disturbance of tsFT101 cells may be due to the diminished level of profilin gene expression.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 6 January 1997 / Received after revision and accepted: 17 April 1997
Rights and permissions
About this article
Cite this article
Cao, Y., Motomura, K., Ohtsuru, A. et al. Profilin gene expression and regulation in a temperature-sensitive breast cancer cell line: tsFT101. Pflügers Arch 434, 341–345 (1997). https://doi.org/10.1007/s004240050406
Issue Date:
DOI: https://doi.org/10.1007/s004240050406