Summary
Reabsorption rates for urate in the loops of Henle were measured in superficial nephrons in the rat 1. under conditions of free flow, 2. using microperfusion and 3. by a microinjection technique. 1. Under conditions of free flow distally measured TF/PUA/TF/PIn-values varied between 0.51 and 0.38 in antidiuretic rats, depending on TF/PIn (UA = both uric acid and urate, In = inulin, TF/P = concentration in tubular fluid to plasma concentration). The corresponding values in samples from end-proximal tubules were 1.06 and in urine 0.19 (U/PUA/U/PIn). 2. In microperfusion experiments of Henle loops early distal recoveries of 2-C14 urate varied between 57 and 86%, depending on the flow rates (10–40 nl/min). 3. In microinjection experiments C14 recovery in urine was about 85% when tracer solution was microinjected into endproximal tubules.
From these results we conclude: 1. The main site of urate reabsorption is located in the loops of Henle. 2. This reabsorption is highly dependent on flow rates. Increase of flow rate through Henle's loop decreases urate reabsorption.
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Partially supported by the “Österreichischer Fond zur Förderung der wissenschaftlichen Forschung”.
Part of this work was presented at the VII. Symp. Ges. Nephr. (1970), Tübingen.
Receiving scholarships from the “Deutsche Forschungsgemeinschaft”.
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Greger, R., Lang, F. & Deetjen, P. Urate handling by the rat kidney. Pflugers Arch. 352, 115–120 (1974). https://doi.org/10.1007/BF00587510
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DOI: https://doi.org/10.1007/BF00587510