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Effect of fermentation conditions on N2 fixation by Methylococcus capsulatus

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Abstract

Nitrogen fixation has been investigated during chemostat fermentations with a culture of Methylococcus capsulatus with natural gas. It is demonstrated that nitrogen fixation occurs under conditions when either nitrate or ammonia as nitrogen source is insufficient for the growth on fixed supply of methane and oxygen. The fixation occurs contrary to expectations within a wide range of dilution rates and with variation of concentration of liquid source of nitrogen. An O2 optimum is determined for the nitrogenase system of the culture in an assay. During fermentation a complete abolishment of nitrogenase reaction is attained at 15% air saturation (dissolved oxygen). Conditions for N2 fixation is unaltered with change of pH from 6.8 to 5.7.

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References

  1. Furigo, A.; Jørgensen, M.H.: Nitrogen limited growth of methanotropic culture. Bioproc. Engin. 9 (1993) 119–127

    Google Scholar 

  2. Murrell, J.X.: The rapid switch-off of nitrogenase activity in obligate methane-oxidizing bacteria. Arch. Microbiol. 150 (1988)

  3. Carlsen, H.N.; H. Jørgensen, L.; Degn, H.: Inhibition by ammonia of the methane utilization in Methylococcus capsulatus (Bath). Appl. Microbiol. Biotechnol. 35 (1991) 124–127

    Google Scholar 

  4. Carlsen, H.N.; H. Jørgensen, L.; Degn, H.: Inhibition by ammonia of the methane oxidation of Methylococcus capsulatus (Bath) by ammonia and methanol. In: Christiansen, C.; Munck, L.; Villadsen, J. (eds): Proc. 5th Europ. Congr. Biotechnol., Copenhagen, 8–13. July 1990, pp 754–757, Copenhagen: Munksgaard 1990

    Google Scholar 

  5. Dalton, H.: Ammonia oxidation by the methane oxidizing bacterium Methylococcus capsulatus strain Bath. Arch. Microbiol. 114 (1977) 273–279

    Google Scholar 

  6. O'Neil, J.G.; Wilkinson, J.F.: Oxidation of ammonia by methaneoxidizing bacteria and the effects of ammonia on methane oxidation. J. Gen. Microbiol. 100 (1977) 407–412

    Google Scholar 

  7. Drozd, J.W.; Godley, A.; Bailey, M.L.: Ammonia oxidation by methane-oxidizing bacteria. Proc. Soc. Gen. Microbiol. 5 (1978) 66–67

    Google Scholar 

  8. Murrell, J.C.; Dalton, H.: Ammonia assimilation in Methylococcus capsulatus (Bath) and other obligate methanotrophs. J. Gen. Microbiol. 129 (1983) 1197–1206

    Google Scholar 

  9. Chen, Y.-P.; Yoch, D.C.: Reconstitution of the electron transport system that couples formate oxidation to nitrogenase in Methylosinus trichosporium OB3b. J. Gen. Microb. 134 (1988) 3123–3128

    Google Scholar 

  10. Murrell, J.C.; Dalton, H.: Nitrogen fixation in obligate methanotrophs. J. Gen. Microbiol. 129 (1983) 3481–3486

    Google Scholar 

  11. Orme-Johnson, W.H.: Molecular basis of biological nitrogen fixation. Ann. Rev. Biophys. Chem. 14 (1985) 419–459

    Google Scholar 

  12. Zhivotchenko, A.G.; Nikonova, E.S.; Jørgensen, M.H.: Copper effect of the growth kinitics of Methylococcus capsulatus (Bath). Biotechnol. Technique 9 (1995), 3, 163–168

    Google Scholar 

  13. Gostomski, P.; Muhlemann, M.; Lin, Y.-H.; Mormino, R.; Bungay, H.: Auxostats for continuous culture research. J. Biotechnol. 37 (1994) 167–177

    Google Scholar 

  14. Mutafov, S.B.; Minkevich, I.G.; Eroshin, V.K.: pH-auxostat: Theory and practice (in Russian). Puschino, 1985

  15. Pohland, H.D.; Huhn, H.J.; Klarman, H.; Prauss, M.; Soyez, K.: High performance bacterial fermentation process with two gaseous substrates. Acta Biotechnol. 12 (1992) 1, 17–25

    Google Scholar 

  16. Dalton, H.; Whittenbury, P.: The acetylene reduction technique as an assay for nitrogenase activity in methane oxidizing bacterium Methylococcus capsulatus strain Bath. Arch. Microbiol. 109 (1976) 147–151

    Google Scholar 

  17. Shah, N.N.; Park, S.; Taylor, R.T.; Droege, M.W.: Cultivation of Methylosinus trichosporium OBSb: III. Production of particulate methane monooxygenase in continuous culture. Biotechnol. Bioeng. (1992) 40, 705–712

    Google Scholar 

  18. Eady, R.E.: The Dinitrogen-Fixing Bacteria. In: Balows, A. et al. (Eds.): The Procaryotes, A Handbook on Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications. 2. edit. Vol. 1, pp 534–553, New York: Springer-Verlag 1992

    Google Scholar 

  19. Pinuev, I.O.: Production of a polysaccharide by Methylobacillus methylophilus and Methylococcus capsulatus under conditions of growth limitation with nitrogen. Prikladn. mikrobiol i biotechnol. (in Russian) 28 (1992) 60–65

    Google Scholar 

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  1. Center for Process Biotechnology, Technical University of Denmark, DK-2800, Lyngby, Denmark

    A. G. Zhivotchenko, E. S. Nikonova & M. H. Jørgensen

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  1. A. G. Zhivotchenko
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  2. E. S. Nikonova
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  3. M. H. Jørgensen
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Zhivotchenko, A.G., Nikonova, E.S. & Jørgensen, M.H. Effect of fermentation conditions on N2 fixation by Methylococcus capsulatus. Bioprocess Engineering 14, 9–15 (1995). https://doi.org/10.1007/BF00369847

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  • DOI: https://doi.org/10.1007/BF00369847

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