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Design and operating criteria for hollow fiber bioreactors

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Abstract

We have analyzed the design and operation of hollow fiber bioreactors for cell culture on the basis of cell growth efficiency and non-uniform fiber spacing. Operating diagrams are presented that describe reactor performance as a function of important operating variables like oxygen concentration and the fiber packing density. The diagrams allow one to find the best set of operating conditions for a fixed reactor design, or to rationally adjust the design parameters for fixed operating conditions.

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Abbreviations

C in :

feed oxygen concentration (mass/volume)

D 2 :

diffusivity of oxygen in continuous cell mass (length/time)

R 2 :

outer radius of hollow fiber (length)

ζ :

packing efficiency (dimensionless)

φ:

Thiele modulus (dimensionless)

Φ:

cellular oxygen consumption rate (mass/volume-time)

References

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Authors and Affiliations

  1. Unisyn Technologies, Inc., 25 South St., 01748, Hopkington, MA.

    C. Sardonini

  2. Department of Chemical Engineering, Worcester Polytechnic Institute, 100 Institute Road, 01609, Worcester, MA

    D. DiBiasio

Authors
  1. C. Sardonini
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  2. D. DiBiasio
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Sardonini, C., DiBiasio, D. Design and operating criteria for hollow fiber bioreactors. Bioprocess Engineering 15, 327–330 (1996). https://doi.org/10.1007/BF02426443

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  • DOI: https://doi.org/10.1007/BF02426443

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