Abstract
Chromatographic effluents were on-line analyzed by Zeeman-ETAAS, using a flow-through cell placed in a graphite furnace autosampler as interface. To obtain high sampling rates, the use of fast graphite furnace programmes was studied. Conventional programmes of 96 s were reduced to 18 s by using hot injection (120 °C) and reducing the charring step to 2 s. The increase of the injection volume from 20 to 60 μl lengthened the programme to 46 s. Nickel had to be added to get a comparable response for both inorganic and organic selenium species (selenite and selenomethionine) and to reduce the interferent effect produced in presence of the chromatographic eluent (TRIS 0.01 mol l-1, NH4NO3 0.1 mol l-1, pH 7). The optimized conditions were applied to the speciation of selenium in human erythrocyte lysates by size exclusion LC-ETAAS. Using a high performance size exclusion column selenium could be assigned to proteins of 100 and 35 kDa. Detection limits in the range of 1 ng (2 μg l-1 for 500 μl injection volume) were obtained for the combined technique.
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Received: 9 October 1996/Revised: 8 July 1996/Accepted: 14 July 1996
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Laborda, F., Vicente, M., Mir, J. et al. Evaluation of on-line coupling size exclusion chromatography electrothermal atomic absorption spectrometry for selenium speciation. Fresenius J Anal Chem 357, 837–843 (1997). https://doi.org/10.1007/s002160050259
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DOI: https://doi.org/10.1007/s002160050259