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Voltage-dependent excitation of motoneurones from spinal locomotor centres in the cat

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Abstract

Lumbar motoneurones were recorded intracellularly during fictive locomotion induced by stimulation of the mesencephalic locomotor region in decerebrate cats. After blocking the action potentials using intracellular QX-314, and by using a discontinuous current clamp, it is shown that the excitatory component of the locomotor drive potentials behaves in a voltage-dependent manner, such that its amplitude increases with depolarisation. As the input to motoneurones during locomotion is comprised of alternating excitation and inhibition, it was desirable to examine the excitatory input in relative isolation. This was accomplished in spinalised decerebrate cats treated with nialamide and l-dihydroxy-phenylalanine (l-DOPA) by studying the excitatory post-synaptic potentials (EPSPs) evoked from the “flexor reflex afferents” (FRA) and extensor Ib afferents, both of which are likely to be mediated via the locomotor network. As expected, these EPSPs also demonstrate a voltage-dependent increase in amplitude. In addition, the input to motoneurones from the network for scratching, which is thought to share interneurones with the locomotor network, also results in voltage-dependent excitation. The possible underlying mechanisms of NMDA-mediated excitation and plateau potentials are discussed:both may contribute to the observed effect. It is suggested that this nonlinear increase in excitation contributes to the mechanisms involved in the production of the high rates of repetitive firing of motoneurones typically seen during locomotion, thus ensuring appropriate muscle contraction.

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Author notes

  1. J. -P. Gossard

    Present address: Department of Physiology, Faculty of Medicine, University of Montréal, C.P. 6128, Succ. A, H3C 3J7, Montréal, Québec, Canada

Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, University of Manitoba, 770 Bannatyne Ave, R3E 0W3, Winnipeg, Manitoba, Canada

    R. M. Brownstone

  2. Department of Medical Physiology, Panum Institute, Copenhagen University, Blegdamsvej 3C, DK-2000, Copenhagen N, Denmark

    J. -P. Gossard & H. Hultborn

Authors
  1. R. M. Brownstone
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  2. J. -P. Gossard
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  3. H. Hultborn
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Brownstone, R.M., Gossard, J.P. & Hultborn, H. Voltage-dependent excitation of motoneurones from spinal locomotor centres in the cat. Exp Brain Res 102, 34–44 (1994). https://doi.org/10.1007/BF00232436

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  • DOI: https://doi.org/10.1007/BF00232436

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