Abstract
NF-κB is found in many neuronal cell types in different states of activity. This study aimed to define which conditions induce constitutive NF-κB activity in cultured hippocampal neurons using activity-specific antibody staining. In co-culture with astroglia, hippocampal neurons were devoid of activated NF-κB. In these co-cultures, NF-κB could not be activated via kainate or glutamate. In contrast, separating neurons from the glial compartment resulted in a time-dependent increase of activated neuronal NF-κB. In this line, activation of NF-κB by kainate or glutamate is very effective in freshly separated cultures, but inhibited when the cultures are reassembled after stimulation. These findings suggests that a neuronal-glial interaction may regulate gene expression via NF-κB.
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Received: 14 June 1999 / Accepted: 8 September 1999
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Kaltschmidt, B., Kaltschmidt, C. Constitutive NF-κB activity is modulated via neuron-astroglia interaction. Exp Brain Res 130, 100–104 (2000). https://doi.org/10.1007/s002210050011
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DOI: https://doi.org/10.1007/s002210050011