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PAPS-cerebroside sulphotransferase activity in developing brain of a neurological mutant of mouse (msd)

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Summary

  1. 1.

    The activity of the sulphotransferase involved in the synthesis of sulphatides, 3′-phosphoadenosine-5′-phosphosulphate cerebroside sulphotransferase (PAPS-CST), was determined in the developing CNS of the myelin synthesis deficiency mutant mouse (msd) and compared to that in control animals. There was a decrease of the PAPS-CST activity in msd mouse in the period from 8 to 26 days. The developmental curve of PAPS-CST activity in msd mice brains essentially paralleled that in normal animals, with a maximum at about 19 days.

  2. 2.

    Basic enzyme properties, such as Km, optimum pH, and heat inactivation, were not affected by the mutation.

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Abbreviations

PAPS-CST:

3′-phosphoadenosine-5′-phosphosulphate-cerebroside sulphotransferase

ATP:

adenosine triphosphate

NFA:

non hydroxy fatty acids

HFA:

hydroxy fatty acids

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Authors and Affiliations

  1. Centre de Neurochimie du C. N. R. S., Strasbourg, France

    L. L. Sarlieve, N. M. Neskovic, G. Rebel & P. Mandel

Authors
  1. L. L. Sarlieve
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  2. N. M. Neskovic
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  3. G. Rebel
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  4. P. Mandel
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Additional information

With the technical assistance of P. Guerin and M. Perraut.

Attaché de Recherche à l'INSERM.

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Sarlieve, L.L., Neskovic, N.M., Rebel, G. et al. PAPS-cerebroside sulphotransferase activity in developing brain of a neurological mutant of mouse (msd). Exp. Brain Res. 19, 158–165 (1974). https://doi.org/10.1007/BF00238532

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  • DOI: https://doi.org/10.1007/BF00238532

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